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Title: Polyfunctional HIV-1 specific response by CD8+ T lymphocytes expressing high levels of CD300a
Authors: Vitallé, Joana
Terrén, Iñigo
Gamboa-Urquijo, Leire
Orrantia, Ane
Tarancón-Díez, Laura
Genebat, Miguel
Leal, Manuel
Ruiz-Mateos, Ezequiel
Borrego, Francisco
Zenarruzabeitia, Olatz
metadata.dc.contributor.authoraffiliation: [Vitallé,J; Terrén,I; Gamboa-Urquijo,L; Orrantia,A; Borrego,F; Zenarruzabeitia,O] Biocruces Bizkaia Health Research Institute, Immunopathology Group, Barakaldo, Spain. [Tarancón-Díez,L; Genebat,M; Leal,M; Ruiz-Mateos,E] Clinic Unit of Infectious Diseases, Microbiology and Preventive Medicine, Institute of Biomedicine of Seville (IBiS), Virgen del Rocío University Hospital, University of Seville, CSIC, Seville, Spain. [Tarancón-Díez,L] Laboratory of Molecular Immuno-Biology, Gregorio Marañón University Hospital, Health Research Institute, Madrid, Spain. [Leal,M] Internal Medicine Service, Santa Ángela de la Cruz Viamed Hospital, Sevilla, Spain. [Borrego,F] Ikerbasque, Basque Foundation for Science, Bilbao, Spain.
Keywords: HIV-1;Cytokines;Peptides;CD300a receptor;T-Lymphocytes;Citocinas;Péptidos;Linfocitos T
metadata.dc.subject.mesh: Medical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Anti-Infective Agents::Antiviral Agents::Anti-Retroviral Agents::Anti-HIV Agents
Medical Subject Headings::Chemicals and Drugs::Biological Factors::Antigens::Antigens, Surface::Antigens, Differentiation::Antigens, CD
Medical Subject Headings::Anatomy::Cells::Blood Cells::Leukocytes::Leukocytes, Mononuclear::Lymphocytes::T-Lymphocytes::CD8-Positive T-Lymphocytes
Medical Subject Headings::Anatomy::Cells::Cells, Cultured
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Intercellular Signaling Peptides and Proteins::Cytokines
Medical Subject Headings::Diseases::Immune System Diseases::Immunologic Deficiency Syndromes::HIV Infections
Medical Subject Headings::Organisms::Viruses::RNA Viruses::Retroviridae::Lentivirus::Lentiviruses, Primate::HIV::HIV-1
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Biochemical Processes::Up-Regulation
Medical Subject Headings::Anatomy::Cells::Blood Cells::Leukocytes::Leukocytes, Mononuclear::Lymphocytes::T-Lymphocytes::T-Lymphocyte Subsets
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides
Issue Date: 8-Apr-2020
Publisher: Springer Nature
Citation: Vitallé J, Terrén I, Gamboa-Urquijo L, Orrantia A, Tarancón-Díez L, Genebat M, et al. Polyfunctional HIV-1 specific response by CD8+ T lymphocytes expressing high levels of CD300a. Sci Rep. 2020 Apr 8;10(1):6070
Abstract: CD300a receptor is found on different CD8+ T cell subsets and its expression has been associated to a more cytotoxic molecular signature. CD300a has an important role in some viral infections and its expression levels are known to be modulated by human immunodeficiency virus (HIV)-1 infection on several cell types. The main objective of this work was to investigate CD300a expression and its regulation during HIV-1 specific CD8+ T cell responses. CD300a receptor expression was analysed by multiparametric flow cytometry on CD8+ T lymphocytes from HIV negative donors, naive HIV-1+ individuals and HIV-1+ subjects under suppressive combined antiretroviral therapy (cART). HIV-1 specific CD8+ T cell response was studied by stimulating cells with HIV-1 derived peptides or with a Gag HIV-1 peptide. Our results showed that HIV-1 specific CD8+ T cells expressing higher levels of CD300a were more polyfunctional showing an increased degranulation and cytokine production. Moreover, we observed an up-regulation of CD300a expression after Gag HIV-1 peptide stimulation. Finally, our results demonstrated an inverse correlation between CD300a expression on CD8+ T lymphocytes and HIV disease progression markers. In conclusion, CD300a expression is associated to a better and more polyfunctional HIV-1 specific CD8+ T cell response.
metadata.dc.identifier.doi: 10.1038/s41598-020-63025-4
ISSN: 2045-2322 (Online)
Appears in Collections:01- Artículos - Hospital Virgen del Rocío
01- Artículos - IBIS. Instituto de Biomedicina de Sevilla

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