RT Journal Article
T1 Polyfunctional HIV-1 specific response by CD8+ T lymphocytes expressing high levels of CD300a
A1 Vitallé, Joana
A1 Terrén, Iñigo
A1 Gamboa-Urquijo, Leire
A1 Orrantia, Ane
A1 Tarancón-Díez, Laura
A1 Genebat, Miguel
A1 Leal, Manuel
A1 Ruiz-Mateos, Ezequiel
A1 Borrego, Francisco
A1 Zenarruzabeitia, Olatz
K1 HIV-1
K1 Cytokines
K1 Peptides
K1 CD300a receptor
K1 T-Lymphocytes
K1 Citocinas
K1 Péptidos
K1 Linfocitos T
AB CD300a receptor is found on different CD8+ T cell subsets and its expression has been associated to a more cytotoxic molecular signature. CD300a has an important role in some viral infections and its expression levels are known to be modulated by human immunodeficiency virus (HIV)-1 infection on several cell types. The main objective of this work was to investigate CD300a expression and its regulation during HIV-1 specific CD8+ T cell responses. CD300a receptor expression was analysed by multiparametric flow cytometry on CD8+ T lymphocytes from HIV negative donors, naive HIV-1+ individuals and HIV-1+ subjects under suppressive combined antiretroviral therapy (cART). HIV-1 specific CD8+ T cell response was studied by stimulating cells with HIV-1 derived peptides or with a Gag HIV-1 peptide. Our results showed that HIV-1 specific CD8+ T cells expressing higher levels of CD300a were more polyfunctional showing an increased degranulation and cytokine production. Moreover, we observed an up-regulation of CD300a expression after Gag HIV-1 peptide stimulation. Finally, our results demonstrated an inverse correlation between CD300a expression on CD8+ T lymphocytes and HIV disease progression markers. In conclusion, CD300a expression is associated to a better and more polyfunctional HIV-1 specific CD8+ T cell response.
PB Springer Nature
YR 2020
FD 2020-04-08
LK http://hdl.handle.net/10668/4112
UL http://hdl.handle.net/10668/4112
LA en
NO Vitallé J, Terrén I, Gamboa-Urquijo L, Orrantia A, Tarancón-Díez L, Genebat M, et al. Polyfunctional HIV-1 specific response by CD8+ T lymphocytes expressing high levels of CD300a. Sci Rep. 2020 Apr 8;10(1):6070
DS RISalud
RD Apr 12, 2025