%0 Journal Article
%A Vitallé, Joana
%A Terrén, Iñigo
%A Gamboa-Urquijo, Leire
%A Orrantia, Ane
%A Tarancón-Díez, Laura
%A Genebat, Miguel
%A Leal, Manuel
%A Ruiz-Mateos, Ezequiel
%A Borrego, Francisco
%A Zenarruzabeitia, Olatz
%T Polyfunctional HIV-1 specific response by CD8+ T lymphocytes expressing high levels of CD300a
%D 2020
%U http://hdl.handle.net/10668/4112
%X CD300a receptor is found on different CD8+ T cell subsets and its expression has been associated to a more cytotoxic molecular signature. CD300a has an important role in some viral infections and its expression levels are known to be modulated by human immunodeficiency virus (HIV)-1 infection on several cell types. The main objective of this work was to investigate CD300a expression and its regulation during HIV-1 specific CD8+ T cell responses. CD300a receptor expression was analysed by multiparametric flow cytometry on CD8+ T lymphocytes from HIV negative donors, naive HIV-1+ individuals and HIV-1+ subjects under suppressive combined antiretroviral therapy (cART). HIV-1 specific CD8+ T cell response was studied by stimulating cells with HIV-1 derived peptides or with a Gag HIV-1 peptide. Our results showed that HIV-1 specific CD8+ T cells expressing higher levels of CD300a were more polyfunctional showing an increased degranulation and cytokine production. Moreover, we observed an up-regulation of CD300a expression after Gag HIV-1 peptide stimulation. Finally, our results demonstrated an inverse correlation between CD300a expression on CD8+ T lymphocytes and HIV disease progression markers. In conclusion, CD300a expression is associated to a better and more polyfunctional HIV-1 specific CD8+ T cell response.
%K HIV-1
%K Cytokines
%K Peptides
%K CD300a receptor
%K T-Lymphocytes
%K Citocinas
%K Péptidos
%K Linfocitos T
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