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Title: Removing Lipemia in Serum/Plasma Samples: A Multicenter Study.
Authors: Castro-Castro, María José
Candás-Estébanez, Beatriz
Esteban-Salán, Margarita
Calmarza, Pilar
Arrobas-Velilla, Teresa
Romero-Román, Carlos
Pocoví-Mieras, Miguel
Aguilar-Doreste, José Ángel
Commission on Lipoprotein and Vascular Diseases, Sociedad Española de Química Clínica
Keywords: 1,1,2-trichlorotrifluoroethane;High-speed centrifugation;Interference;Lipemia;Lipid removal method;LipoClear
metadata.dc.subject.mesh: Alanine Transaminase
Laboratories, Hospital
Liquid-Liquid Extraction
Issue Date: 2018
Abstract: Lipemia, a significant source of analytical errors in clinical laboratory settings, should be removed prior to measuring biochemical parameters. We investigated whether lipemia in serum/plasma samples can be removed using a method that is easier and more practicable than ultracentrifugation, the current reference method. Seven hospital laboratories in Spain participated in this study. We first compared the effectiveness of ultracentrifugation (108,200×g) and high-speed centrifugation (10,000×g for 15 minutes) in removing lipemia. Second, we compared high-speed centrifugation with two liquid-liquid extraction methods-LipoClear (StatSpin, Norwood, USA), and 1,1,2-trichlorotrifluoroethane (Merck, Darmstadt, Germany). We assessed 14 biochemical parameters: serum/plasma concentrations of sodium ion, potassium ion, chloride ion, glucose, total protein, albumin, creatinine, urea, alkaline phosphatase, gamma-glutamyl transferase, alanine aminotransferase, aspartate-aminotransferase, calcium, and bilirubin. We analyzed whether the differences between lipemia removal methods exceeded the limit for clinically significant interference (LCSI). When ultracentrifugation and high-speed centrifugation were compared, no parameter had a difference that exceeded the LCSI. When high-speed centrifugation was compared with the two liquid-liquid extraction methods, we found differences exceeding the LCSI in protein, calcium, and aspartate aminotransferase in the comparison with 1,1,2-trichlorotrifluoroethane, and in protein, albumin, and calcium in the comparison with LipoClear. Differences in other parameters did not exceed the LCSI. High-speed centrifugation (10,000×g for 15 minutes) can be used instead of ultracentrifugation to remove lipemia in serum/plasma samples. LipoClear and 1,1,2-trichlorotrifluoroethane are unsuitable as they interfere with the measurement of certain parameters.
metadata.dc.identifier.doi: 10.3343/alm.2018.38.6.518
Appears in Collections:Producción 2020

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