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Title: | Removing Lipemia in Serum/Plasma Samples: A Multicenter Study. |
Authors: | Castro-Castro, María José Candás-Estébanez, Beatriz Esteban-Salán, Margarita Calmarza, Pilar Arrobas-Velilla, Teresa Romero-Román, Carlos Pocoví-Mieras, Miguel Aguilar-Doreste, José Ángel Commission on Lipoprotein and Vascular Diseases, Sociedad Española de Química Clínica |
Keywords: | 1,1,2-trichlorotrifluoroethane;High-speed centrifugation;Interference;Lipemia;Lipid removal method;LipoClear |
metadata.dc.subject.mesh: | Alanine Transaminase Calcium Centrifugation Creatinine Humans Hyperlipidemias Laboratories, Hospital Lipids Liquid-Liquid Extraction |
Issue Date: | 2018 |
Abstract: | Lipemia, a significant source of analytical errors in clinical laboratory settings, should be removed prior to measuring biochemical parameters. We investigated whether lipemia in serum/plasma samples can be removed using a method that is easier and more practicable than ultracentrifugation, the current reference method. Seven hospital laboratories in Spain participated in this study. We first compared the effectiveness of ultracentrifugation (108,200×g) and high-speed centrifugation (10,000×g for 15 minutes) in removing lipemia. Second, we compared high-speed centrifugation with two liquid-liquid extraction methods-LipoClear (StatSpin, Norwood, USA), and 1,1,2-trichlorotrifluoroethane (Merck, Darmstadt, Germany). We assessed 14 biochemical parameters: serum/plasma concentrations of sodium ion, potassium ion, chloride ion, glucose, total protein, albumin, creatinine, urea, alkaline phosphatase, gamma-glutamyl transferase, alanine aminotransferase, aspartate-aminotransferase, calcium, and bilirubin. We analyzed whether the differences between lipemia removal methods exceeded the limit for clinically significant interference (LCSI). When ultracentrifugation and high-speed centrifugation were compared, no parameter had a difference that exceeded the LCSI. When high-speed centrifugation was compared with the two liquid-liquid extraction methods, we found differences exceeding the LCSI in protein, calcium, and aspartate aminotransferase in the comparison with 1,1,2-trichlorotrifluoroethane, and in protein, albumin, and calcium in the comparison with LipoClear. Differences in other parameters did not exceed the LCSI. High-speed centrifugation (10,000×g for 15 minutes) can be used instead of ultracentrifugation to remove lipemia in serum/plasma samples. LipoClear and 1,1,2-trichlorotrifluoroethane are unsuitable as they interfere with the measurement of certain parameters. |
URI: | http://hdl.handle.net/10668/12730 |
metadata.dc.identifier.doi: | 10.3343/alm.2018.38.6.518 |
Appears in Collections: | Producción 2020 |
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