%0 Journal Article
%A Castro-Castro, María José
%A Candás-Estébanez, Beatriz
%A Esteban-Salán, Margarita
%A Calmarza, Pilar
%A Arrobas-Velilla, Teresa
%A Romero-Román, Carlos
%A Pocoví-Mieras, Miguel
%A Aguilar-Doreste, José Ángel
%A Commission on Lipoprotein and Vascular Diseases, Sociedad Española de Química Clínica
%T Removing Lipemia in Serum/Plasma Samples: A Multicenter Study.
%D 2018
%U http://hdl.handle.net/10668/12730
%X Lipemia, a significant source of analytical errors in clinical laboratory settings, should be removed prior to measuring biochemical parameters. We investigated whether lipemia in serum/plasma samples can be removed using a method that is easier and more practicable than ultracentrifugation, the current reference method. Seven hospital laboratories in Spain participated in this study. We first compared the effectiveness of ultracentrifugation (108,200×g) and high-speed centrifugation (10,000×g for 15 minutes) in removing lipemia. Second, we compared high-speed centrifugation with two liquid-liquid extraction methods-LipoClear (StatSpin, Norwood, USA), and 1,1,2-trichlorotrifluoroethane (Merck, Darmstadt, Germany). We assessed 14 biochemical parameters: serum/plasma concentrations of sodium ion, potassium ion, chloride ion, glucose, total protein, albumin, creatinine, urea, alkaline phosphatase, gamma-glutamyl transferase, alanine aminotransferase, aspartate-aminotransferase, calcium, and bilirubin. We analyzed whether the differences between lipemia removal methods exceeded the limit for clinically significant interference (LCSI). When ultracentrifugation and high-speed centrifugation were compared, no parameter had a difference that exceeded the LCSI. When high-speed centrifugation was compared with the two liquid-liquid extraction methods, we found differences exceeding the LCSI in protein, calcium, and aspartate aminotransferase in the comparison with 1,1,2-trichlorotrifluoroethane, and in protein, albumin, and calcium in the comparison with LipoClear. Differences in other parameters did not exceed the LCSI. High-speed centrifugation (10,000×g for 15 minutes) can be used instead of ultracentrifugation to remove lipemia in serum/plasma samples. LipoClear and 1,1,2-trichlorotrifluoroethane are unsuitable as they interfere with the measurement of certain parameters.
%K 1,1,2-trichlorotrifluoroethane
%K High-speed centrifugation
%K Interference
%K Lipemia
%K Lipid removal method
%K LipoClear
%~