RT Journal Article
T1 Removing Lipemia in Serum/Plasma Samples: A Multicenter Study.
A1 Castro-Castro, María José
A1 Candás-Estébanez, Beatriz
A1 Esteban-Salán, Margarita
A1 Calmarza, Pilar
A1 Arrobas-Velilla, Teresa
A1 Romero-Román, Carlos
A1 Pocoví-Mieras, Miguel
A1 Aguilar-Doreste, José Ángel
A1 Commission on Lipoprotein and Vascular Diseases, Sociedad Española de Química Clínica
K1 1,1,2-trichlorotrifluoroethane
K1 High-speed centrifugation
K1 Interference
K1 Lipemia
K1 Lipid removal method
K1 LipoClear
AB Lipemia, a significant source of analytical errors in clinical laboratory settings, should be removed prior to measuring biochemical parameters. We investigated whether lipemia in serum/plasma samples can be removed using a method that is easier and more practicable than ultracentrifugation, the current reference method. Seven hospital laboratories in Spain participated in this study. We first compared the effectiveness of ultracentrifugation (108,200×g) and high-speed centrifugation (10,000×g for 15 minutes) in removing lipemia. Second, we compared high-speed centrifugation with two liquid-liquid extraction methods-LipoClear (StatSpin, Norwood, USA), and 1,1,2-trichlorotrifluoroethane (Merck, Darmstadt, Germany). We assessed 14 biochemical parameters: serum/plasma concentrations of sodium ion, potassium ion, chloride ion, glucose, total protein, albumin, creatinine, urea, alkaline phosphatase, gamma-glutamyl transferase, alanine aminotransferase, aspartate-aminotransferase, calcium, and bilirubin. We analyzed whether the differences between lipemia removal methods exceeded the limit for clinically significant interference (LCSI). When ultracentrifugation and high-speed centrifugation were compared, no parameter had a difference that exceeded the LCSI. When high-speed centrifugation was compared with the two liquid-liquid extraction methods, we found differences exceeding the LCSI in protein, calcium, and aspartate aminotransferase in the comparison with 1,1,2-trichlorotrifluoroethane, and in protein, albumin, and calcium in the comparison with LipoClear. Differences in other parameters did not exceed the LCSI. High-speed centrifugation (10,000×g for 15 minutes) can be used instead of ultracentrifugation to remove lipemia in serum/plasma samples. LipoClear and 1,1,2-trichlorotrifluoroethane are unsuitable as they interfere with the measurement of certain parameters.
YR 2018
FD 2018
LK http://hdl.handle.net/10668/12730
UL http://hdl.handle.net/10668/12730
LA en
DS RISalud
RD Apr 11, 2025