Publication: The Analysis of Platelet-Derived circRNA Repertoire as Potential Diagnostic Biomarker for Non-Small Cell Lung Cancer.
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Date
2021-09-13
Authors
D'Ambrosi, Silvia
Visser, Allerdien
Antunes-Ferreira, Mafalda
Poutsma, Ankie
Giannoukakos, Stavros
Sol, Nik
Sabrkhany, Siamack
Bahce, Idris
Kuijpers, Marijke J E
Oude Egbrink, Mirjam G A
Advisors
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Volume Title
Publisher
MDPI AG
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Abstract
Tumor-educated Platelets (TEPs) have emerged as rich biosources of cancer-related RNA profiles in liquid biopsies applicable for cancer detection. Although human blood platelets have been found to be enriched in circular RNA (circRNA), no studies have investigated the potential of circRNA as platelet-derived biomarkers for cancer. In this proof-of-concept study, we examine whether the circRNA signature of blood platelets can be used as a liquid biopsy biomarker for the detection of non-small cell lung cancer (NSCLC). We analyzed the total RNA, extracted from the platelet samples collected from NSCLC patients and asymptomatic individuals, using RNA sequencing (RNA-Seq). Identification and quantification of known and novel circRNAs were performed using the accurate CircRNA finder suite (ACFS), followed by the differential transcript expression analysis using a modified version of our thromboSeq software. Out of 4732 detected circRNAs, we identified 411 circRNAs that are significantly (p-value
Description
MeSH Terms
Humans
RNA
RNA, Circular
Carcinoma, Non-Small-Cell Lung
RNA-Seq
Reverse Transcription
Down-Regulation
Nuclear Receptor Interacting Protein 1
Blood Platelets
Lung Neoplasms
Gene Expression Profiling
Sequence Analysis, RNA
Software
Polymerase Chain Reaction
RNA
RNA, Circular
Carcinoma, Non-Small-Cell Lung
RNA-Seq
Reverse Transcription
Down-Regulation
Nuclear Receptor Interacting Protein 1
Blood Platelets
Lung Neoplasms
Gene Expression Profiling
Sequence Analysis, RNA
Software
Polymerase Chain Reaction
DeCS Terms
ARN
ARN Circular
Análisis de secuencia de ARN
Carcinoma de pulmón decélulas no pequeñas
Humanos
Neoplasias pulmonares
Perfilación de la expresión génica plaquetas
Programas informáticos
Proteína de interacción con receptores nucleares 1
RNA-Seq
Reacción en cadena de la polimerasa
ARN Circular
Análisis de secuencia de ARN
Carcinoma de pulmón decélulas no pequeñas
Humanos
Neoplasias pulmonares
Perfilación de la expresión génica plaquetas
Programas informáticos
Proteína de interacción con receptores nucleares 1
RNA-Seq
Reacción en cadena de la polimerasa
CIE Terms
Keywords
biomarkers, circular RNA, liquid biopsy, non-small cell lung cancer, platelets
Citation
D'Ambrosi S, Visser A, Antunes-Ferreira M, Poutsma A, Giannoukakos S, Sol N, et al. The Analysis of Platelet-Derived circRNA Repertoire as Potential Diagnostic Biomarker for Non-Small Cell Lung Cancer. Cancers (Basel). 2021 Sep 16;13(18):4644.