%0 Journal Article
%A D'Ambrosi, Silvia
%A Visser, Allerdien
%A Antunes-Ferreira, Mafalda
%A Poutsma, Ankie
%A Giannoukakos, Stavros
%A Sol, Nik
%A Sabrkhany, Siamack
%A Bahce, Idris
%A Kuijpers, Marijke J E
%A Oude Egbrink, Mirjam G A
%A Griffioen, Arjan W
%A Best, Myron G
%A Koppers-Lalic, Danijela
%A Oudejans, Cees
%A Würdinger, Thomas
%T The Analysis of Platelet-Derived circRNA Repertoire as Potential Diagnostic Biomarker for Non-Small Cell Lung Cancer.
%D 2021
%@ 2072-6694
%U http://hdl.handle.net/10668/18559
%X Tumor-educated Platelets (TEPs) have emerged as rich biosources of cancer-related RNA profiles in liquid biopsies applicable for cancer detection. Although human blood platelets have been found to be enriched in circular RNA (circRNA), no studies have investigated the potential of circRNA as platelet-derived biomarkers for cancer. In this proof-of-concept study, we examine whether the circRNA signature of blood platelets can be used as a liquid biopsy biomarker for the detection of non-small cell lung cancer (NSCLC). We analyzed the total RNA, extracted from the platelet samples collected from NSCLC patients and asymptomatic individuals, using RNA sequencing (RNA-Seq). Identification and quantification of known and novel circRNAs were performed using the accurate CircRNA finder suite (ACFS), followed by the differential transcript expression analysis using a modified version of our thromboSeq software. Out of 4732 detected circRNAs, we identified 411 circRNAs that are significantly (p-value
%K biomarkers
%K circular RNA
%K liquid biopsy
%K non-small cell lung cancer
%K platelets
%~