Publication: Osteoarticular Expression of Musashi-1 in an Experimental Model of Arthritis.
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Identifiers
Date
2015
Authors
O'Valle, Francisco
Peregrina, Magdalena
Crespo-Lora, Vicente
Galindo-Moreno, Pablo
Roman, Maria
Padial-Molina, Miguel
Mesa, Francisco
Aneiros-Fernandez, Jose
Aguilar, David
Gonzalez-Rey, Elena
Advisors
Journal Title
Journal ISSN
Volume Title
Publisher
Hindawi Publishing Corporation
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Abstract
Background. Collagen-induced arthritis (CIA), a murine experimental disease model induced by immunization with type II collagen (CII), is used to evaluate novel therapeutic strategies for rheumatoid arthritis. Adult stem cell marker Musashi-1 (Msi1) plays an important role in regulating the maintenance and differentiation of stem/precursor cells. The objectives of this investigation were to perform a morphological study of the experimental CIA model, evaluate the effect of TNFα-blocker (etanercept) treatment, and determine the immunohistochemical expression of Msi1 protein. Methods. CIA was induced in 50 male DBA1/J mice for analyses of tissue and serum cytokine; clinical and morphological lesions in limbs; and immunohistochemical expression of Msi1. Results. Clinically, TNFα-blocker treatment attenuated CIA on day 32 after immunization (P < 0.001). Msi1 protein expression was significantly higher in joints damaged by CIA than in those with no lesions (P < 0.0001) and was related to the severity of the lesions (Spearman's rho = 0.775, P = 0.0001). Conclusions. Treatment with etanercept attenuates osteoarticular lesions in the murine CIA model. Osteoarticular expression of Msi1 protein is increased in joints with CIA-induced lesion and absent in nonlesioned joints, suggesting that this protein is expressed when the lesion is produced in order to favor tissue repair.
Description
Journal Article;
MeSH Terms
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Models, Theoretical
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Models, Animal::Disease Models, Animal::Arthritis, Experimental
Medical Subject Headings::Chemicals and Drugs::Macromolecular Substances::Polymers::Biopolymers::Collagen
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice
Medical Subject Headings::Anatomy::Cells::Stem Cells::Adult Stem Cells
Medical Subject Headings::Chemicals and Drugs::Macromolecular Substances::Polymers::Biopolymers::Collagen::Fibrillar Collagens::Collagen Type II
Medical Subject Headings::Health Care::Health Care Facilities, Manpower, and Services::Health Services::Preventive Health Services::Primary Prevention::Immunization
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Serum Globulins::Immunoglobulins::Antibodies::Immunoglobulin Isotypes::Immunoglobulin G
Medical Subject Headings::Anatomy::Musculoskeletal System::Skeleton::Joints
Medical Subject Headings::Organisms::Eukaryota::Animals
Medical Subject Headings::Check Tags::Male
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides::Intercellular Signaling Peptides and Proteins::Cytokines::Tumor Necrosis Factors::Tumor Necrosis Factor-alpha
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Membrane Proteins::Receptors, Cell Surface::Receptors, Immunologic::Receptors, Cytokine::Receptors, Tumor Necrosis Factor
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Models, Animal::Disease Models, Animal::Arthritis, Experimental
Medical Subject Headings::Chemicals and Drugs::Macromolecular Substances::Polymers::Biopolymers::Collagen
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice
Medical Subject Headings::Anatomy::Cells::Stem Cells::Adult Stem Cells
Medical Subject Headings::Chemicals and Drugs::Macromolecular Substances::Polymers::Biopolymers::Collagen::Fibrillar Collagens::Collagen Type II
Medical Subject Headings::Health Care::Health Care Facilities, Manpower, and Services::Health Services::Preventive Health Services::Primary Prevention::Immunization
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Serum Globulins::Immunoglobulins::Antibodies::Immunoglobulin Isotypes::Immunoglobulin G
Medical Subject Headings::Anatomy::Musculoskeletal System::Skeleton::Joints
Medical Subject Headings::Organisms::Eukaryota::Animals
Medical Subject Headings::Check Tags::Male
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides::Intercellular Signaling Peptides and Proteins::Cytokines::Tumor Necrosis Factors::Tumor Necrosis Factor-alpha
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Membrane Proteins::Receptors, Cell Surface::Receptors, Immunologic::Receptors, Cytokine::Receptors, Tumor Necrosis Factor
DeCS Terms
CIE Terms
Keywords
Modelos teóricos, Artritis experimental, Colágeno tipo II, Ratones, Células madre adultas, Inmunización, Inmunoglobulina g, Articulaciones, Animales, Masculino, Factor de necrosis tumoral alfa, Receptores del factor de necrosis tumoral
Citation
O'Valle F, Peregrina M, Crespo-Lora V, Galindo-Moreno P, Roman M, Padial-Molina M, et al. Osteoarticular Expression of Musashi-1 in an Experimental Model of Arthritis. Biomed Res Int. 2015:681456