%0 Journal Article
%A O'Valle, Francisco
%A Peregrina, Magdalena
%A Crespo-Lora, Vicente
%A Galindo-Moreno, Pablo
%A Roman, Maria
%A Padial-Molina, Miguel
%A Mesa, Francisco
%A Aneiros-Fernandez, Jose
%A Aguilar, David
%A Gonzalez-Rey, Elena
%A Delgado, Mario
%A Hernandez-Cortes, Pedro
%T Osteoarticular Expression of Musashi-1 in an Experimental Model of Arthritis.
%D 2015
%U http://hdl.handle.net/10668/2097
%X Background. Collagen-induced arthritis (CIA), a murine experimental disease model induced by immunization with type II collagen (CII), is used to evaluate novel therapeutic strategies for rheumatoid arthritis. Adult stem cell marker Musashi-1 (Msi1) plays an important role in regulating the maintenance and differentiation of stem/precursor cells. The objectives of this investigation were to perform a morphological study of the experimental CIA model, evaluate the effect of TNFα-blocker (etanercept) treatment, and determine the immunohistochemical expression of Msi1 protein. Methods. CIA was induced in 50 male DBA1/J mice for analyses of tissue and serum cytokine; clinical and morphological lesions in limbs; and immunohistochemical expression of Msi1. Results. Clinically, TNFα-blocker treatment attenuated CIA on day 32 after immunization (P < 0.001). Msi1 protein expression was significantly higher in joints damaged by CIA than in those with no lesions (P < 0.0001) and was related to the severity of the lesions (Spearman's rho = 0.775, P = 0.0001). Conclusions. Treatment with etanercept attenuates osteoarticular lesions in the murine CIA model. Osteoarticular expression of Msi1 protein is increased in joints with CIA-induced lesion and absent in nonlesioned joints, suggesting that this protein is expressed when the lesion is produced in order to favor tissue repair.
%K Modelos teóricos
%K Artritis experimental
%K Colágeno tipo II
%K Ratones
%K Células madre adultas
%K Inmunización
%K Inmunoglobulina g
%K Articulaciones
%K Animales
%K Masculino
%K Factor de necrosis tumoral alfa
%K Receptores del factor de necrosis tumoral
%~