Publication: Real-time molecular epidemiology of tuberculosis by direct genotyping of smear-positive clinical specimens.
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Identifiers
Date
2012-05
Authors
Alonso, María
Herranz, Marta
Martínez Lirola, Miguel
González-Rivera, Milagros
Bouza, Emilio
García de Viedma, Darío
Advisors
Journal Title
Journal ISSN
Volume Title
Publisher
American Society for Microbiology
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Abstract
We applied MIRU-VNTR (mycobacterial interspersed repetitive-unit-variable-number tandem-repeat typing) to directly analyze the bacilli present in 61 stain-positive specimens from tuberculosis patients. A complete MIRU type (24 loci) was obtained for all but one (no amplification in one locus) of the specimens (98.4%), and the allelic values fully correlated with those obtained from the corresponding cultures. Our study is the first to demonstrate that real-time genotyping of Mycobacterium tuberculosis can be achieved, fully transforming the way in which molecular epidemiology techniques can be integrated into control programs.
Description
Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't;
MeSH Terms
Medical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::DNA::DNA, Bacterial
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Molecular Typing
Medical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Gram-Positive Rods::Gram-Positive Asporogenous Rods::Gram-Positive Asporogenous Rods, Regular::Mycobacteriaceae::Mycobacterium::Mycobacterium tuberculosis
Medical Subject Headings::Diseases::Respiratory Tract Diseases::Respiratory Tract Infections::Tuberculosis, Pulmonary
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Molecular Structure::Base Sequence::Repetitive Sequences, Nucleic Acid::Tandem Repeat Sequences::Minisatellite Repeats
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Molecular Epidemiology
Medical Subject Headings::Anatomy::Fluids and Secretions::Bodily Secretions::Sputum
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Molecular Typing
Medical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Gram-Positive Rods::Gram-Positive Asporogenous Rods::Gram-Positive Asporogenous Rods, Regular::Mycobacteriaceae::Mycobacterium::Mycobacterium tuberculosis
Medical Subject Headings::Diseases::Respiratory Tract Diseases::Respiratory Tract Infections::Tuberculosis, Pulmonary
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Molecular Structure::Base Sequence::Repetitive Sequences, Nucleic Acid::Tandem Repeat Sequences::Minisatellite Repeats
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Molecular Epidemiology
Medical Subject Headings::Anatomy::Fluids and Secretions::Bodily Secretions::Sputum
DeCS Terms
CIE Terms
Keywords
ADN bacteriano, Tipificación molecular, Esputo, Tuberculosis pulmonar
Citation
Alonso M, Herranz M, Martínez Lirola M, González-Rivera M, Bouza E, García de Viedma D. Real-time molecular epidemiology of tuberculosis by direct genotyping of smear-positive clinical specimens. J. Clin. Microbiol.. 2012 May; 50(5):1755-7