%0 Journal Article
%A Alonso, María
%A Herranz, Marta
%A Martínez Lirola, Miguel
%A González-Rivera, Milagros
%A Bouza, Emilio
%A García de Viedma, Darío
%T Real-time molecular epidemiology of tuberculosis by direct genotyping of smear-positive clinical specimens.
%D 2012
%@ 0095-1137
%U http://hdl.handle.net/10668/2018
%X We applied MIRU-VNTR (mycobacterial interspersed repetitive-unit-variable-number tandem-repeat typing) to directly analyze the bacilli present in 61 stain-positive specimens from tuberculosis patients. A complete MIRU type (24 loci) was obtained for all but one (no amplification in one locus) of the specimens (98.4%), and the allelic values fully correlated with those obtained from the corresponding cultures. Our study is the first to demonstrate that real-time genotyping of Mycobacterium tuberculosis can be achieved, fully transforming the way in which molecular epidemiology techniques can be integrated into control programs.
%K ADN bacteriano
%K Tipificación molecular
%K Esputo
%K Tuberculosis pulmonar
%~