RT Journal Article
T1 Real-time molecular epidemiology of tuberculosis by direct genotyping of smear-positive clinical specimens.
A1 Alonso, María
A1 Herranz, Marta
A1 Martínez Lirola, Miguel
A1 González-Rivera, Milagros
A1 Bouza, Emilio
A1 García de Viedma, Darío
K1 ADN bacteriano
K1 Tipificación molecular
K1 Esputo
K1 Tuberculosis pulmonar
AB We applied MIRU-VNTR (mycobacterial interspersed repetitive-unit-variable-number tandem-repeat typing) to directly analyze the bacilli present in 61 stain-positive specimens from tuberculosis patients. A complete MIRU type (24 loci) was obtained for all but one (no amplification in one locus) of the specimens (98.4%), and the allelic values fully correlated with those obtained from the corresponding cultures. Our study is the first to demonstrate that real-time genotyping of Mycobacterium tuberculosis can be achieved, fully transforming the way in which molecular epidemiology techniques can be integrated into control programs.
PB American Society for Microbiology
SN 0095-1137
YR 2012
FD 2012-05
LK http://hdl.handle.net/10668/2018
UL http://hdl.handle.net/10668/2018
LA en
NO Alonso M, Herranz M, Martínez Lirola M, González-Rivera M, Bouza E, García de Viedma D. Real-time molecular epidemiology of tuberculosis by direct genotyping of smear-positive clinical specimens. J. Clin. Microbiol.. 2012 May; 50(5):1755-7
NO Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't;
DS RISalud
RD Apr 7, 2025