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Real-time molecular epidemiology of tuberculosis by direct genotyping of smear-positive clinical specimens.

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dc.contributor.authorAlonso, María
dc.contributor.authorHerranz, Marta
dc.contributor.authorMartínez Lirola, Miguel
dc.contributor.authorGonzález-Rivera, Milagros
dc.contributor.authorBouza, Emilio
dc.contributor.authorGarcía de Viedma, Darío
dc.contributor.authoraffiliation[Alonso,M; Herranz,M; Bouza,E; García de Viedma,D] Hosp Gen Univ Gregorio Marañon, Serv Microbiol & Enfermedades Infecciosas, Madrid, Spain. [Alonso,M; Herranz,M; Bouza, E; García de Viedma,D; González-Rivera,M ] Inst Invest Sanitaria Gregorio Marañon, Madrid, Spain. [Alonso,M; Herranz,M; Bouza,E; García de Viedma,D] CIBER Enfermedades Respiratorias. CIBERES.Spain.[Martínez Lirola,M] Complejo Hosp Torrecárdenas,Almería,Spain.es
dc.contributor.funderMaría Alonso is supported by a contract (REF CA09/00054) from the Instituto de Salud Carlos III (Fondo de Investigaciones Sanitarias) and provides technical support in the Unidad Central de Análisis Molecular of Hospital General Universitario Gregorio Marañón. LightCycler 2.0 was acquired with a grant (REF IF08/36173) from Instituto de Salud Carlos III (Fondo de Investigaciones Sanitarias). The 3130xl Genetic Analyzer was partially financed by grants from Fondo de Investigaciones Sanitarias (IF01-3624, IF08-36173). The study was partially financed by Fondo de Investigaciones Sanitarias (S09/02205), Junta de Andalucía (PI-0444/2008 and PI-0306-2009), and SEPAR (763-09).
dc.date.accessioned2015-10-15T09:15:54Z
dc.date.available2015-10-15T09:15:54Z
dc.date.issued2012-05
dc.descriptionEvaluation Studies; Journal Article; Research Support, Non-U.S. Gov't;es
dc.description.abstractWe applied MIRU-VNTR (mycobacterial interspersed repetitive-unit-variable-number tandem-repeat typing) to directly analyze the bacilli present in 61 stain-positive specimens from tuberculosis patients. A complete MIRU type (24 loci) was obtained for all but one (no amplification in one locus) of the specimens (98.4%), and the allelic values fully correlated with those obtained from the corresponding cultures. Our study is the first to demonstrate that real-time genotyping of Mycobacterium tuberculosis can be achieved, fully transforming the way in which molecular epidemiology techniques can be integrated into control programs.es
dc.description.versionYeses
dc.identifier.citationAlonso M, Herranz M, Martínez Lirola M, González-Rivera M, Bouza E, García de Viedma D. Real-time molecular epidemiology of tuberculosis by direct genotyping of smear-positive clinical specimens. J. Clin. Microbiol.. 2012 May; 50(5):1755-7es
dc.identifier.doi10.1128/JCM.00132-12
dc.identifier.essn1098-660X
dc.identifier.issn0095-1137
dc.identifier.pmid22378907
dc.identifier.urihttp://hdl.handle.net/10668/2018
dc.journal.titleJournal of clinical microbiology
dc.language.isoen
dc.publisherAmerican Society for Microbiologyes
dc.relation.publisherversionhttp://jcm.asm.org/content/50/5/1755.longes
dc.rights.accessRightsopen access
dc.subjectADN bacterianoes
dc.subjectTipificación moleculares
dc.subjectEsputoes
dc.subjectTuberculosis pulmonares
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::DNA::DNA, Bacteriales
dc.subject.meshMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Molecular Typinges
dc.subject.meshMedical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Gram-Positive Rods::Gram-Positive Asporogenous Rods::Gram-Positive Asporogenous Rods, Regular::Mycobacteriaceae::Mycobacterium::Mycobacterium tuberculosises
dc.subject.meshMedical Subject Headings::Diseases::Respiratory Tract Diseases::Respiratory Tract Infections::Tuberculosis, Pulmonaryes
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Molecular Structure::Base Sequence::Repetitive Sequences, Nucleic Acid::Tandem Repeat Sequences::Minisatellite Repeatses
dc.subject.meshMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humanses
dc.subject.meshMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Molecular Epidemiologyes
dc.subject.meshMedical Subject Headings::Anatomy::Fluids and Secretions::Bodily Secretions::Sputumes
dc.titleReal-time molecular epidemiology of tuberculosis by direct genotyping of smear-positive clinical specimens.es
dc.typeresearch article
dc.type.hasVersionVoR
dspace.entity.typePublication

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