Publication: Conventional Matrices Loaded Onto a Graphene Layer Enhances MALDI-TOF/TOF Signal: Its Application to Improve Detection of Phosphorylated Peptides.
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2015-11-30
Authors
Rodriguez, Carlos E
Palacios, Javier
Fajardo, Ignacio
Urdiales, Jose Luis
Le Guevel, Xavier
Lozano, Jose
Sanchez-Jimenez, Francisca
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Abstract
This is the first study where graphene is used as a MALDI adjuvant in combination with the traditional matrix α-cyano-4-hydroxycinnamic acid (CHCA) to improve the signal intensity of peptide samples. Use of this amended matrix not only leads to increased signals but also to a higher number of peaks detected in complex samples. Additionally, the use of graphene has a stabilizing effect that can also be exploited to improve the detection of easily cleavable molecules.
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MeSH Terms
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Peptides
Phosphoproteins
Phosphorylation
Signal Processing, Computer-Assisted
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Peptides
Phosphoproteins
Phosphorylation
Signal Processing, Computer-Assisted
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Diagnóstico
Grafito
Péptidos
Ácidos cumáricos
Grafito
Péptidos
Ácidos cumáricos
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Keywords
Graphene, MALDI-TOF/TOF, Phosphorylated peptides
Citation
Rodríguez CE, Palacios J, Fajardo I, Urdiales JL, Le Guével X, Lozano J, et al. Conventional Matrices Loaded Onto a Graphene Layer Enhances MALDI-TOF/TOF Signal: Its Application to Improve Detection of Phosphorylated Peptides. J Am Soc Mass Spectrom. 2016 Feb;27(2):366-9.