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Conventional Matrices Loaded Onto a Graphene Layer Enhances MALDI-TOF/TOF Signal: Its Application to Improve Detection of Phosphorylated Peptides.

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dc.contributor.authorRodriguez, Carlos E
dc.contributor.authorPalacios, Javier
dc.contributor.authorFajardo, Ignacio
dc.contributor.authorUrdiales, Jose Luis
dc.contributor.authorLe Guevel, Xavier
dc.contributor.authorLozano, Jose
dc.contributor.authorSanchez-Jimenez, Francisca
dc.contributor.funderSCBI Proteomics Unit of the University of Málaga (Parque Tecnológico de Andalucía)
dc.date.accessioned2023-01-25T08:30:20Z
dc.date.available2023-01-25T08:30:20Z
dc.date.issued2015-11-30
dc.description.abstractThis is the first study where graphene is used as a MALDI adjuvant in combination with the traditional matrix α-cyano-4-hydroxycinnamic acid (CHCA) to improve the signal intensity of peptide samples. Use of this amended matrix not only leads to increased signals but also to a higher number of peaks detected in complex samples. Additionally, the use of graphene has a stabilizing effect that can also be exploited to improve the detection of easily cleavable molecules.
dc.description.sponsorshipThe SCBI Proteomics Unit of the University of Málaga (Parque Tecnológico de Andalucía) enabled the present work to be carried out. It was supported by grants SAF2011-26518 (MINECO, Spain), SAF2010-20203 (MINECO Spain), and CVI-06585 (PAIDI, Andalusia). CIBERER is an initiative of Institute de Salud Carlos III. The authors thankfully acknowledge the expertise, understanding, and assistance of the Nanotech Unit of the SCBI, for sharing support and encouragement
dc.identifier.citationRodríguez CE, Palacios J, Fajardo I, Urdiales JL, Le Guével X, Lozano J, et al. Conventional Matrices Loaded Onto a Graphene Layer Enhances MALDI-TOF/TOF Signal: Its Application to Improve Detection of Phosphorylated Peptides. J Am Soc Mass Spectrom. 2016 Feb;27(2):366-9.
dc.identifier.doi10.1007/s13361-015-1299-3
dc.identifier.essn1879-1123
dc.identifier.pmid26620529
dc.identifier.unpaywallURLhttps://hal.archives-ouvertes.fr/hal-02338004/file/Conventional%20Matrices%20Loaded%20Onto%20a%20Graphene%20Layer%20Enhances%20MALDI-TOF_TOF%20Signal-%20Its%20Application%20to%20Improve%20Detection%20of%20Phosphorylated%20Peptides.pdf
dc.identifier.urihttp://hdl.handle.net/10668/9645
dc.issue.number2
dc.journal.titleJournal of the American Society for Mass Spectrometry
dc.journal.titleabbreviationJ Am Soc Mass Spectrom
dc.language.isoen
dc.organizationCentro Andaluz de Nanomedicina y Biotecnología-BIONAND
dc.organizationInstituto de Investigación Biomédica de Málaga-IBIMA
dc.organizationHospital Universitario Virgen de la Victoria
dc.page.number4
dc.provenanceRealizada la curación de contenido 23/08/2024
dc.pubmedtypeJournal Article
dc.relation.projectIDSAF2011-26518
dc.relation.projectIDSAF2010-20203
dc.relation.projectIDCVI-06585
dc.rights.accessRightsopen access
dc.subjectGraphene
dc.subjectMALDI-TOF/TOF
dc.subjectPhosphorylated peptides
dc.subject.decsDiagnóstico
dc.subject.decsGrafito
dc.subject.decsPéptidos
dc.subject.decsÁcidos cumáricos
dc.subject.meshGraphite
dc.subject.meshPeptides
dc.subject.meshPhosphoproteins
dc.subject.meshPhosphorylation
dc.subject.meshSignal Processing, Computer-Assisted
dc.subject.meshSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
dc.titleConventional Matrices Loaded Onto a Graphene Layer Enhances MALDI-TOF/TOF Signal: Its Application to Improve Detection of Phosphorylated Peptides.
dc.typeresearch article
dc.type.hasVersionVOR
dc.volume.number27
dspace.entity.typePublication

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