RT Journal Article
T1 Conventional Matrices Loaded Onto a Graphene Layer Enhances MALDI-TOF/TOF Signal: Its Application to Improve Detection of Phosphorylated Peptides.
A1 Rodriguez, Carlos E
A1 Palacios, Javier
A1 Fajardo, Ignacio
A1 Urdiales, Jose Luis
A1 Le Guevel, Xavier
A1 Lozano, Jose
A1 Sanchez-Jimenez, Francisca
K1 Graphene
K1 MALDI-TOF/TOF
K1 Phosphorylated peptides
AB This is the first study where graphene is used as a MALDI adjuvant in combination with the traditional matrix α-cyano-4-hydroxycinnamic acid (CHCA) to improve the signal intensity of peptide samples. Use of this amended matrix not only leads to increased signals but also to a higher number of peaks detected in complex samples. Additionally, the use of graphene has a stabilizing effect that can also be exploited to improve the detection of easily cleavable molecules. 
YR 2015
FD 2015-11-30
LK http://hdl.handle.net/10668/9645
UL http://hdl.handle.net/10668/9645
LA en
NO Rodríguez CE, Palacios J, Fajardo I, Urdiales JL, Le Guével X, Lozano J, et al. Conventional Matrices Loaded Onto a Graphene Layer Enhances MALDI-TOF/TOF Signal: Its Application to Improve Detection of Phosphorylated Peptides. J Am Soc Mass Spectrom. 2016 Feb;27(2):366-9.
NO The SCBI Proteomics Unit of the University of Málaga (Parque Tecnológico de Andalucía) enabled the present work to be carried out. It was supported by grants SAF2011-26518 (MINECO, Spain), SAF2010-20203 (MINECO Spain), and CVI-06585 (PAIDI, Andalusia). CIBERER is an initiative of Institute de Salud Carlos III. The authors thankfully acknowledge the expertise,understanding, and assistance of the Nanotech Unit of the SCBI,for sharing support and encouragement
DS RISalud
RD Apr 6, 2025