Diagnostic pre-screening method based on N-gene dropout or delay to increase feasibility of SARS-CoV-2 VOC B.1.1.7 detection.
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2021-07-16
Authors
Sánchez-Calvo, Juan M
Alados Arboledas, Juan C
Ros Vidal, Luis
de Francisco, José L
López Prieto, María D
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Abstract
To compare the RT-PCR Allplex SARS-CoV-2/FluA/FluB/RSV Assay (Allplex assay) with other methods of detection of VOC B.1.1.7. Suspected and non-suspected cases of VOC B.1.1.7 were defined according to the VirSNiP assay, which detects N501Y and deletion H69-V70. For pre-screening, the Allplex™ and TaqPath assays were used. One hundred and sixteen suspected and 113 non-suspected cases were included. In the suspected cases, the Allplex assay showed N-gene dropout, or delayed Ct values of 6.27 ± 1.21 and 6.66 ± 1.41 compared with those of the RdRP and S-gene target, respectively. Agreement between the Allplex and TaqPath assays was 100% when the RdRP and S-gene targets had Ct values
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MeSH Terms
COVID-19
Coronavirus Nucleocapsid Proteins
Feasibility Studies
Gene Expression Regulation, Viral
Genome, Viral
Humans
Phosphoproteins
SARS-CoV-2
Sensitivity and Specificity
Whole Genome Sequencing
Coronavirus Nucleocapsid Proteins
Feasibility Studies
Gene Expression Regulation, Viral
Genome, Viral
Humans
Phosphoproteins
SARS-CoV-2
Sensitivity and Specificity
Whole Genome Sequencing
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CIE Terms
Keywords
2019-nCoV RT-PCR diagnostic panel, Mutation, SARS-CoV-2, Screening Assays, Variant, Whole-genome sequencing