RT Journal Article
T1 Diagnostic pre-screening method based on N-gene dropout or delay to increase feasibility of SARS-CoV-2 VOC B.1.1.7 detection.
A1 Sánchez-Calvo, Juan M
A1 Alados Arboledas, Juan C
A1 Ros Vidal, Luis
A1 de Francisco, José L
A1 López Prieto, María D
K1 2019-nCoV RT-PCR diagnostic panel
K1 Mutation
K1 SARS-CoV-2
K1 Screening Assays
K1 Variant
K1 Whole-genome sequencing
AB To compare the RT-PCR Allplex SARS-CoV-2/FluA/FluB/RSV Assay (Allplex assay) with other methods of detection of VOC B.1.1.7. Suspected and non-suspected cases of VOC B.1.1.7 were defined according to the VirSNiP assay, which detects N501Y and deletion H69-V70. For pre-screening, the Allplex™ and TaqPath assays were used. One hundred and sixteen suspected and 113 non-suspected cases were included. In the suspected cases, the Allplex assay showed N-gene dropout, or delayed Ct values of 6.27 ± 1.21 and 6.66 ± 1.41 compared with those of the RdRP and S-gene target, respectively. Agreement between the Allplex and TaqPath assays was 100% when the RdRP and S-gene targets had Ct values
YR 2021
FD 2021-07-16
LK https://hdl.handle.net/10668/25028
UL https://hdl.handle.net/10668/25028
LA en
DS RISalud
RD Apr 8, 2025