Diagnostic pre-screening method based on N-gene dropout or delay to increase feasibility of SARS-CoV-2 VOC B.1.1.7 detection.

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dc.contributor.authorSánchez-Calvo, Juan M
dc.contributor.authorAlados Arboledas, Juan C
dc.contributor.authorRos Vidal, Luis
dc.contributor.authorde Francisco, José L
dc.contributor.authorLópez Prieto, María D
dc.date.accessioned2025-01-07T12:54:01Z
dc.date.available2025-01-07T12:54:01Z
dc.date.issued2021-07-16
dc.description.abstractTo compare the RT-PCR Allplex SARS-CoV-2/FluA/FluB/RSV Assay (Allplex assay) with other methods of detection of VOC B.1.1.7. Suspected and non-suspected cases of VOC B.1.1.7 were defined according to the VirSNiP assay, which detects N501Y and deletion H69-V70. For pre-screening, the Allplex™ and TaqPath assays were used. One hundred and sixteen suspected and 113 non-suspected cases were included. In the suspected cases, the Allplex assay showed N-gene dropout, or delayed Ct values of 6.27 ± 1.21 and 6.66 ± 1.41 compared with those of the RdRP and S-gene target, respectively. Agreement between the Allplex and TaqPath assays was 100% when the RdRP and S-gene targets had Ct values
dc.identifier.doi10.1016/j.diagmicrobio.2021.115491
dc.identifier.essn1879-0070
dc.identifier.pmcPMC8282941
dc.identifier.pmid34464903
dc.identifier.pubmedURLhttps://pmc.ncbi.nlm.nih.gov/articles/PMC8282941/pdf
dc.identifier.unpaywallURLhttps://doi.org/10.1016/j.diagmicrobio.2021.115491
dc.identifier.urihttps://hdl.handle.net/10668/25028
dc.issue.number4
dc.journal.titleDiagnostic microbiology and infectious disease
dc.journal.titleabbreviationDiagn Microbiol Infect Dis
dc.language.isoen
dc.organizationSAS - Hospital Universitario de Jerez de la Frontera
dc.page.number115491
dc.pubmedtypeJournal Article
dc.rights.accessRightsopen access
dc.subject2019-nCoV RT-PCR diagnostic panel
dc.subjectMutation
dc.subjectSARS-CoV-2
dc.subjectScreening Assays
dc.subjectVariant
dc.subjectWhole-genome sequencing
dc.subject.meshCOVID-19
dc.subject.meshCoronavirus Nucleocapsid Proteins
dc.subject.meshFeasibility Studies
dc.subject.meshGene Expression Regulation, Viral
dc.subject.meshGenome, Viral
dc.subject.meshHumans
dc.subject.meshPhosphoproteins
dc.subject.meshSARS-CoV-2
dc.subject.meshSensitivity and Specificity
dc.subject.meshWhole Genome Sequencing
dc.titleDiagnostic pre-screening method based on N-gene dropout or delay to increase feasibility of SARS-CoV-2 VOC B.1.1.7 detection.
dc.typeresearch article
dc.type.hasVersionVoR
dc.volume.number101

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