Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/474
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dc.contributor.authorArilla, M C-
dc.contributor.authorIbarrola, I-
dc.contributor.authorMartínez, A-
dc.contributor.authorMonteseirín, J-
dc.contributor.authorConde, J-
dc.contributor.authorAsturias, J A-
dc.date.accessioned2012-09-14T11:27:44Z-
dc.date.available2012-09-14T11:27:44Z-
dc.date.issued2008-05-01-
dc.identifier.citationArilla MC, Ibarrola I, Martínez A, Monteseirín J, Conde J, Asturias JA. An antibody-based ELISA for quantification of Ani s 1, a major allergen from Anisakis simplex. Parasitology. 2008;135(6):735-40es
dc.identifier.issn1469-8161 (Print)-
dc.identifier.issn0031-1820 (Online)-
dc.identifier.urihttp://hdl.handle.net/10668/474-
dc.description.abstractAnisakis simplex is a nematode parasite that can infect humans who have eaten raw or undercooked seafood. Larvae invading the gastrointestinal mucosa excrete/secrete proteins that are implicated in the pathogenesis of anisakiasis and can induce IgE-mediated symptoms. Since Ani s 1 is a potent secreted allergen with important clinical relevance, its measurement could assess the quality of allergenic products used in diagnosis/immunotherapy of Anisakis allergy and track the presence of A. simplex parasites in fish foodstuffs. An antibody-based ELISA for quantification of Ani s 1 has been developed based on monoclonal antibody 4F2 as capture antibody and biotin-labelled polyclonal antibodies against Ani s 1 as detection reagent. The dose-response standard curves, obtained with natural and recombinant antigens, ranged from 4 to 2000 ng/ml and were identical and parallel to that of the A. simplex extract. The linear portion of the dose-response curve with nAni s 1 was between 15 and 250 ng/ml with inter-assay and intra-assays coefficients of variation less than 20% and 10%, respectively. The assay was specific since there was no cross-reaction with other extracts (except Ascaris extracts) and was highly sensitive (detection limit of 1·8 ng/ml), being able to detect Ani s 1 in fish extracts from codfish and monkfish.es
dc.description.sponsorshipThis work was supported by Bial-Arístegui and by Grants FIT-090100-2007-76 from the Programa Nacional de Biomedicina (Acción PROFARMA, Ministerio de Industria, Turismo y Comercio, Spain) and IT-2006/0000480 from the Programa INNOTEK Departamento de Industria, Comercio y Turismo, Gobierno Vasco). J.M. is under the Programa de Intensificación de la Actividad Investigadora del Sistema Nacional de Salud.es
dc.language.isoenes
dc.publisherCambridge University Presses
dc.relation.ispartofParasitologyes
dc.subjectAnisakis simplexes
dc.subjectAnisakis allergyes
dc.subjectQuantificationes
dc.subjectMonoclonal antibodyes
dc.subjectImmunoassayes
dc.subjectFood allergyes
dc.subjectRecombinant antigenes
dc.subjectParasite detectiones
dc.subjectAlergia a Anisakises
dc.subjectCuantificaciónes
dc.subjectAnticuerpo monoclonales
dc.subjectInmunoensayoes
dc.subjectAlergia a los alimentoses
dc.subjectAntígeno recombinantees
dc.subjectDetección de parásitoses
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Biological Factors::Antigens::Allergenses
dc.subject.meshMedical Subject Headings::Diseases::Parasitic Diseases::Intestinal Diseases, Parasitic::Anisakiasises
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Immunoproteins::Immunoglobulins::Antibodies::Antibodies, Helminthes
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Carrier Proteins::Calcium-Binding Proteinses
dc.subject.meshMedical Subject Headings::Anatomy::Cells::Cells, Cultured::Cell Line::Cell Line, Tumores
dc.subject.meshMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Immunologic Techniques::Immunologic Tests::Immunoassay::Immunoenzyme Techniques::Enzyme-Linked Immunosorbent Assayes
dc.subject.meshMedical Subject Headings::Diseases::Animal Diseases::Fish Diseaseses
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Helminth Proteinses
dc.subject.meshMedical Subject Headings::Anatomy::Cells::Cells, Cultured::Clone Cells::Hybridomases
dc.subject.meshMedical Subject Headings::Organisms::Eukaryota::Animals::Animal Population Groups::Animals, Laboratory::Animals, Inbred Strains::Mice, Inbred Strains::Mice, Inbred BALB Ces
dc.subject.meshMedical Subject Headings::Organisms::Eukaryota::Animals::Invertebrates::Molluscaes
dc.subject.meshMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Statistics as Topic::Sensitivity and Specificityes
dc.subject.meshMedical Subject Headings::Anatomy::Hemic and Immune Systems::Immune System::Lymphatic System::Lymphoid Tissue::Spleenes
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Biological Factors::Antigens::Allergenses
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Immunoproteins::Immunoglobulins::Antibodies::Antibodies, Helminthes
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Helminth Proteinses
dc.subject.meshMedical Subject Headings::Organisms::Eukaryota::Animals::Invertebrates::Helminths::Nematoda::Secernentea::Ascaridida::Ascaridoidea::Anisakises
dc.subject.meshMedical Subject Headings::Organisms::Eukaryota::Animals::Invertebrates::Arthropods::Crustaceaes
dc.titleAn antibody-based ELISA for quantification of Ani s 1, a major allergen from Anisakis simplex.es
dc.typeinfo:eu-repo/semantics/articlees
dc.description.versionYeses
dc.identifier.pmid18377696-
dc.rights.accessRightsAcceso abiertoes
dc.identifier.doi10.1017/S0031182008004332-
dc.type.versioninfo:eu-repo/semantics/publishedes
dc.relation.publisherversionhttp://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=1886152es
dc.contributor.authoraffiliation[Arilla,MC;Ibarrola,I;Martínez,A;Asturias,JA]Research & Development Department,Bial-Arístegui, Bilbao, Spain.[Monteseirín,J] Department of Medicine, Immunology and Allergy Service, Virgen Macarena University Hospital, Sevilla.[Monteseirín,J;Conde,J] Sagrado Corazón Clinic, Sevilla, Spaines
dc.type.subtypeArtículoes
Appears in Collections:01- Artículos - Hospital Virgen Macarena

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