RT Journal Article
T1 An antibody-based ELISA for quantification of Ani s 1, a major allergen from Anisakis simplex.
A1 Arilla, M C
A1 Ibarrola, I
A1 Martínez, A
A1 Monteseirín, J
A1 Conde, J
A1 Asturias, J A
K1 Anisakis simplex
K1 Anisakis allergy
K1 Quantification
K1 Monoclonal antibody
K1 Immunoassay
K1 Food allergy
K1 Recombinant antigen
K1 Parasite detection
K1 Alergia a Anisakis
K1 Cuantificación
K1 Anticuerpo monoclonal
K1 Inmunoensayo
K1 Alergia a los alimentos
K1 Antígeno recombinante
K1 Detección de parásitos
AB Anisakis simplex is a nematode parasite that can infect humans who have eaten raw or undercooked seafood. Larvae invading the gastrointestinal mucosa excrete/secrete proteins that are implicated in the pathogenesis of anisakiasis and can induce IgE-mediated symptoms. Since Ani s 1 is a potent secreted allergen with important clinical relevance, its measurement could assess the quality of allergenic products used in diagnosis/immunotherapy of Anisakis allergy and track the presence of A. simplex parasites in fish foodstuffs. An antibody-based ELISA for quantification of Ani s 1 has been developed based on monoclonal antibody 4F2 as capture antibody and biotin-labelled polyclonal antibodies against Ani s 1 as detection reagent. The dose-response standard curves, obtained with natural and recombinant antigens, ranged from 4 to 2000 ng/ml and were identical and parallel to that of the A. simplex extract. The linear portion of the dose-response curve with nAni s 1 was between 15 and 250 ng/ml with inter-assay and intra-assays coefficients of variation less than 20% and 10%, respectively. The assay was specific since there was no cross-reaction with other extracts (except Ascaris extracts) and was highly sensitive (detection limit of 1·8 ng/ml), being able to detect Ani s 1 in fish extracts from codfish and monkfish.
PB Cambridge University Press
SN 1469-8161
YR 2008
FD 2008-05-01
LK http://hdl.handle.net/10668/474
UL http://hdl.handle.net/10668/474
LA en
NO Arilla MC, Ibarrola I, Martínez A, Monteseirín J, Conde J, Asturias JA. An antibody-based ELISA for quantification of Ani s 1, a major allergen from Anisakis simplex. Parasitology. 2008;135(6):735-40
DS RISalud
RD Apr 5, 2025