%0 Journal Article
%A Arilla, M C
%A Ibarrola, I
%A Martínez, A
%A Monteseirín, J
%A Conde, J
%A Asturias, J A
%T An antibody-based ELISA for quantification of Ani s 1, a major allergen from Anisakis simplex.
%D 2008
%@ 1469-8161
%U http://hdl.handle.net/10668/474
%X Anisakis simplex is a nematode parasite that can infect humans who have eaten raw or undercooked seafood. Larvae invading the gastrointestinal mucosa excrete/secrete proteins that are implicated in the pathogenesis of anisakiasis and can induce IgE-mediated symptoms. Since Ani s 1 is a potent secreted allergen with important clinical relevance, its measurement could assess the quality of allergenic products used in diagnosis/immunotherapy of Anisakis allergy and track the presence of A. simplex parasites in fish foodstuffs. An antibody-based ELISA for quantification of Ani s 1 has been developed based on monoclonal antibody 4F2 as capture antibody and biotin-labelled polyclonal antibodies against Ani s 1 as detection reagent. The dose-response standard curves, obtained with natural and recombinant antigens, ranged from 4 to 2000 ng/ml and were identical and parallel to that of the A. simplex extract. The linear portion of the dose-response curve with nAni s 1 was between 15 and 250 ng/ml with inter-assay and intra-assays coefficients of variation less than 20% and 10%, respectively. The assay was specific since there was no cross-reaction with other extracts (except Ascaris extracts) and was highly sensitive (detection limit of 1·8 ng/ml), being able to detect Ani s 1 in fish extracts from codfish and monkfish.
%K Anisakis simplex
%K Anisakis allergy
%K Quantification
%K Monoclonal antibody
%K Immunoassay
%K Food allergy
%K Recombinant antigen
%K Parasite detection
%K Alergia a Anisakis
%K Cuantificación
%K Anticuerpo monoclonal
%K Inmunoensayo
%K Alergia a los alimentos
%K Antígeno recombinante
%K Detección de parásitos
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