Publication: Biased and Unbiased Methods for the Detection of Off-Target Cleavage by CRISPR/Cas9: An Overview
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Date
2016-09-01
Authors
Martin, Francisco
Sanchez-Hernandez, Sabina
Gutierrez-Guerrero, Alejandra
Pinedo-Gomez, Javier
Benabdellah, Karim
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Mdpi
Abstract
The clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein 9 endonuclease (Cas9) derived from bacterial adaptive immune systems is a revolutionary tool used in both basic and applied science. It is a versatile system that enables the genome of different species to be modified by generating double strand breaks (DSBs) at specific locations. However, all of the CRISPR/Cas9 systems can also produce DSBs at off-target sites that differ substantially from on-target sites. The generation of DSBs in locations outside the intended site can produce mutations that need to be carefully monitored, especially when using these tools for therapeutic purposes. However, off-target analyses of the CRISPR/Cas9 system have been very challenging, particularly when performed directly in cells. In this manuscript, we review the different strategies developed to identify off-targets generated by CRISPR/cas9 systems and other specific nucleases (ZFNs, TALENs) in real target cells.
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Keywords
specific nucleases, CRISPR, cas9, off-target sites, real target cells, unbiased, biased, CHIPSeq, IDLVs, GUIDE-seq, LAM-HTGTS, Genome-wide analysis, Chip-seq, Crispr-cas9, Cas9, Repeats, Sites, Mutations, Nucleases, Talens, Genes