RT Generic
T1 Biased and Unbiased Methods for the Detection of Off-Target Cleavage by CRISPR/Cas9: An Overview
A1 Martin, Francisco
A1 Sanchez-Hernandez, Sabina
A1 Gutierrez-Guerrero, Alejandra
A1 Pinedo-Gomez, Javier
A1 Benabdellah, Karim
K1 specific nucleases
K1 CRISPR
K1 cas9
K1 off-target sites
K1 real target cells
K1 unbiased
K1 biased
K1 CHIPSeq
K1 IDLVs
K1 GUIDE-seq
K1 LAM-HTGTS
K1 Genome-wide analysis
K1 Chip-seq
K1 Crispr-cas9
K1 Cas9
K1 Repeats
K1 Sites
K1 Mutations
K1 Nucleases
K1 Talens
K1 Genes
AB The clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein 9 endonuclease (Cas9) derived from bacterial adaptive immune systems is a revolutionary tool used in both basic and applied science. It is a versatile system that enables the genome of different species to be modified by generating double strand breaks (DSBs) at specific locations. However, all of the CRISPR/Cas9 systems can also produce DSBs at off-target sites that differ substantially from on-target sites. The generation of DSBs in locations outside the intended site can produce mutations that need to be carefully monitored, especially when using these tools for therapeutic purposes. However, off-target analyses of the CRISPR/Cas9 system have been very challenging, particularly when performed directly in cells. In this manuscript, we review the different strategies developed to identify off-targets generated by CRISPR/cas9 systems and other specific nucleases (ZFNs, TALENs) in real target cells.
PB Mdpi
SN 1422-0067
YR 2016
FD 2016-09-01
LK http://hdl.handle.net/10668/19271
UL http://hdl.handle.net/10668/19271
LA en
DS RISalud
RD Apr 8, 2025