Publication: UV-fixed-thick-blotch preparation improves sensitivity of auramine staining.
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Date
2013-10
Authors
Cejudo-García, M A
Muñoz-Davila, M J
Martínez-Lirola, M J
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Publisher
American Society for Microbiology
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Abstract
We describe here a very simple modification of the auramine staining procedure based on preparation of a UV-fixed thick blotch which allowed us to reach an overall sensitivity of 0.82 (592 acid-fast bacillus [AFB]-positive specimens/722 initial respiratory specimens with positive mycobacterial culture) and sensitivities of 0.93 (526 AFB-positive specimens/564 culture-positive specimens) for Mycobacterium tuberculosis complex and 0.42 (66 AFB-positive specimens/158 culture-positive specimens) for nontuberculous mycobacteria.
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MeSH Terms
Medical Subject Headings::Diseases::Bacterial Infections and Mycoses::Bacterial Infections::Gram-Positive Bacterial Infections::Actinomycetales Infections::Mycobacterium Infections::Tuberculosis
Medical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Gram-Positive Rods::Gram-Positive Asporogenous Rods::Gram-Positive Asporogenous Rods, Regular::Mycobacteriaceae::Mycobacterium
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Microbiological Techniques::Bacteriological Techniques
Medical Subject Headings::Chemicals and Drugs::Organic Chemicals::Amines::Aniline Compounds::Benzophenoneidum
Medical Subject Headings::Anatomy::Fluids and Secretions::Bodily Secretions::Sputum
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Specimen Handling
Medical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Gram-Positive Rods::Gram-Positive Asporogenous Rods::Gram-Positive Asporogenous Rods, Regular::Mycobacteriaceae::Mycobacterium
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Microbiological Techniques::Bacteriological Techniques
Medical Subject Headings::Chemicals and Drugs::Organic Chemicals::Amines::Aniline Compounds::Benzophenoneidum
Medical Subject Headings::Anatomy::Fluids and Secretions::Bodily Secretions::Sputum
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Specimen Handling
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Keywords
Tuberculosis, Mycobacterium, Técnicas bacteriológicas, Benzofenoneido, Esputo, Manejo de especímenes
Citation
Cejudo-García MA, Muñoz-Davila MJ, Martínez-Lirola MJ. UV-fixed-thick-blotch preparation improves sensitivity of auramine staining. J. Clin. Microbiol.. 2013 ; 51(10):3400-2