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UV-fixed-thick-blotch preparation improves sensitivity of auramine staining.

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dc.contributor.authorCejudo-García, M A
dc.contributor.authorMuñoz-Davila, M J
dc.contributor.authorMartínez-Lirola, M J
dc.contributor.authoraffiliation[Cejudo-García,MA; Muñoz-Davila,MJ; Martínez-Lirola,MJ] Microbiology Laboratory, Biotechnology Clinic Management Unit, Torrecárdenas Hospital Complex, Almería, Spaines
dc.date.accessioned2015-10-26T12:56:25Z
dc.date.available2015-10-26T12:56:25Z
dc.date.issued2013-10
dc.description.abstractWe describe here a very simple modification of the auramine staining procedure based on preparation of a UV-fixed thick blotch which allowed us to reach an overall sensitivity of 0.82 (592 acid-fast bacillus [AFB]-positive specimens/722 initial respiratory specimens with positive mycobacterial culture) and sensitivities of 0.93 (526 AFB-positive specimens/564 culture-positive specimens) for Mycobacterium tuberculosis complex and 0.42 (66 AFB-positive specimens/158 culture-positive specimens) for nontuberculous mycobacteria.es
dc.description.versionYeses
dc.identifier.citationCejudo-García MA, Muñoz-Davila MJ, Martínez-Lirola MJ. UV-fixed-thick-blotch preparation improves sensitivity of auramine staining. J. Clin. Microbiol.. 2013 ; 51(10):3400-2es
dc.identifier.doi10.1128/JCM.00974-13
dc.identifier.essn1098-660X
dc.identifier.issn0095-1137
dc.identifier.pmcPMC3811656
dc.identifier.pmid23863571
dc.identifier.urihttp://hdl.handle.net/10668/2036
dc.journal.titleJournal of Clinical Microbiology
dc.language.isoes
dc.publisherAmerican Society for Microbiologyes
dc.relation.publisherversionhttp://jcm.asm.org/content/51/10/3400.abstractes
dc.rights.accessRightsopen access
dc.subjectTuberculosises
dc.subjectMycobacteriumes
dc.subjectTécnicas bacteriológicases
dc.subjectBenzofenoneidoes
dc.subjectEsputoes
dc.subjectManejo de especímeneses
dc.subject.meshMedical Subject Headings::Diseases::Bacterial Infections and Mycoses::Bacterial Infections::Gram-Positive Bacterial Infections::Actinomycetales Infections::Mycobacterium Infections::Tuberculosises
dc.subject.meshMedical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Gram-Positive Rods::Gram-Positive Asporogenous Rods::Gram-Positive Asporogenous Rods, Regular::Mycobacteriaceae::Mycobacteriumes
dc.subject.meshMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Microbiological Techniques::Bacteriological Techniqueses
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Organic Chemicals::Amines::Aniline Compounds::Benzophenoneidumes
dc.subject.meshMedical Subject Headings::Anatomy::Fluids and Secretions::Bodily Secretions::Sputumes
dc.subject.meshMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Specimen Handlinges
dc.titleUV-fixed-thick-blotch preparation improves sensitivity of auramine staining.es
dc.typeresearch article
dc.type.hasVersionVoR
dspace.entity.typePublication

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