RT Journal Article T1 UV-fixed-thick-blotch preparation improves sensitivity of auramine staining. A1 Cejudo-García, M A A1 Muñoz-Davila, M J A1 Martínez-Lirola, M J K1 Tuberculosis K1 Mycobacterium K1 Técnicas bacteriológicas K1 Benzofenoneido K1 Esputo K1 Manejo de especímenes AB We describe here a very simple modification of the auramine staining procedure based on preparation of a UV-fixed thick blotch which allowed us to reach an overall sensitivity of 0.82 (592 acid-fast bacillus [AFB]-positive specimens/722 initial respiratory specimens with positive mycobacterial culture) and sensitivities of 0.93 (526 AFB-positive specimens/564 culture-positive specimens) for Mycobacterium tuberculosis complex and 0.42 (66 AFB-positive specimens/158 culture-positive specimens) for nontuberculous mycobacteria. PB American Society for Microbiology SN 0095-1137 YR 2013 FD 2013-10 LK http://hdl.handle.net/10668/2036 UL http://hdl.handle.net/10668/2036 LA es NO Cejudo-García MA, Muñoz-Davila MJ, Martínez-Lirola MJ. UV-fixed-thick-blotch preparation improves sensitivity of auramine staining. J. Clin. Microbiol.. 2013 ; 51(10):3400-2 DS RISalud RD Apr 7, 2025