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Selenium, selenoproteins and selenometabolites in mothers and babies at the time of birth.

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Date

2017-05-23

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Santos, Cristina
Garcia-Fuentes, Eduardo
Callejón-Leblic, Belen
Garcia-Barrera, Tamara
Gomez-Ariza, Jose Luis
Rayman, Margaret P
Velasco, Ines

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Cambridge University Press
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Abstract

The deficiency of Se, an essential micronutrient, has been implicated in adverse pregnancy outcomes. Our study was designed to determine total serum Se, selenoproteins (extracellular glutathione peroxidase (GPx-3), selenoprotein P (SeP)), selenoalbumin (SeAlb) and selenometabolites in healthy women and their newborns at delivery. This cross-sectional study included eighty-three healthy mother-baby couples. Total Se and Se species concentrations were measured in maternal and umbilical cord sera by an in-series coupling of two-dimensional size-exclusion and affinity HPLC. Additional measurements of serum SeP concentration and of serum GPx-3 enzyme activity were carried out using ELISA. Total Se concentration was significantly higher in maternal serum than in cord serum (68·9 (sd 15·2) and 56·1 (sd 14·6) µg/l, respectively; P<0·01). There were significant correlations between selenoprotein and SeAlb concentrations in mothers and newborns, although they also showed significant differences in GPx-3 (11·2(SD 3·7) v.10·5(SD 3·5)µg/l; P<0·01), SeP (42·5(SD 9·5) v.28·1 (SD 7·7)µg/l; P<0·01) and SeAlb (11·6(SD 3·6) v.14·1(SD 4·3)µg/l; P<0·01) concentrations in maternal and cord sera, respectively. Serum GPx-3 activity and concentration were positively correlated in mothers (r 0·33; P=0·038) but not in newborns. GPx-3 activity in cord serum was significantly correlated with gestational age (r 0·44; P=0·009). SeAlb concentration was significantly higher in babies, whereas SeP and GPx-3 concentrations were significantly higher in mothers. The differences cannot be explained by simple diffusion; specific transfer mechanisms are probably involved. GPx-3 concentrations in mothers, at delivery, are related to maternal Se status, whereas the GPx-3 activity in cord serum depends on gestational age.

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MeSH Terms

Adult
Cross-Sectional Studies
Enzyme-Linked Immunosorbent Assay
Female
Fetal Blood
Glutathione Peroxidase
Humans
Infant, Newborn
Postpartum Period
Pregnancy
Selenoproteins
Young Adult

DeCS Terms

Edad gestacional
Selenoproteínas
Composición familiar
Micronutrientes
Cromatografía líquida de alta presión
Ensayo de inmunoadsorción enzimática

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Keywords

2D/SE-AF-HPLC two-dimensional size-exclusion and affinity HPLC, GPx-3 extracellular glutathione peroxidase, SeAlb selenoalbumin, SeMetab selenometabolites, SeP selenoprotein P, Gestation, Selenium, Selenoprotein P, Selenoproteins, Área de Gestión Sanitaria Norte de Huelva

Citation

Santos C, García-Fuentes E, Callejón-Leblic B, García-Barrera T, Gómez-Ariza JL, Rayman MP, et al. Selenium, selenoproteins and selenometabolites in mothers and babies at the time of birth. Br J Nutr. 2017 May;117(9):1304-1311