Publication: CENP-V is required for proper chromosome segregation through interaction with spindle microtubules in mouse oocytes
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Identifiers
Date
2021-11-11
Authors
Nabi, Dalileh
Drechsler, Hauke
Pschirer, Johannes
Korn, Franz
Schuler, Nadine
Diez, Stefan
Jessberger, Rolf
Chacón, Mariola
Advisors
Journal Title
Journal ISSN
Volume Title
Publisher
Springer Nature
Abstract
Proper chromosome segregation is essential to avoid aneuploidy, yet this process fails with increasing age in mammalian oocytes. Here we report a role for the scarcely described protein CENP-V in oocyte spindle formation and chromosome segregation. We show that depending on the oocyte maturation state, CENP-V localizes to centromeres, to microtubule organizing centers, and to spindle microtubules. We find that Cenp-V-/- oocytes feature severe deficiencies, including metaphase I arrest, strongly reduced polar body extrusion, increased numbers of mis-aligned chromosomes and aneuploidy, multipolar spindles, unfocused spindle poles and loss of kinetochore spindle fibres. We also show that CENP-V protein binds, diffuses along, and bundles microtubules in vitro. The spindle assembly checkpoint arrests about half of metaphase I Cenp-V-/- oocytes from young adults only. This finding suggests checkpoint weakening in ageing oocytes, which mature despite carrying mis-aligned chromosomes. Thus, CENP-V is a microtubule bundling protein crucial to faithful oocyte meiosis, and Cenp-V-/- oocytes reveal age-dependent weakening of the spindle assembly checkpoint.
Description
MeSH Terms
Medical Subject Headings::Organisms::Eukaryota::Animals
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Cell Division::Cell Nucleus Division::Chromosome Segregation
Medical Subject Headings::Check Tags::Female
Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Cycle::Cell Cycle Checkpoints::M Phase Cell Cycle Checkpoints
Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Cycle::Cell Division::Cell Nucleus Division::Meiosis
Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Cycle::Cell Division::Cell Nucleus Division::Metaphase
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice
Medical Subject Headings::Anatomy::Cells::Cellular Structures::Intracellular Space::Cytoplasm::Cytoplasmic Structures::Cytoskeleton::Microtubule-Organizing Center
Medical Subject Headings::Anatomy::Cells::Cellular Structures::Intracellular Space::Cytoplasm::Cytoplasmic Structures::Cytoskeleton::Microtubules
Medical Subject Headings::Anatomy::Cells::Germ Cells::Ovum::Oocytes
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Cell Division::Cell Nucleus Division::Chromosome Segregation
Medical Subject Headings::Check Tags::Female
Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Cycle::Cell Cycle Checkpoints::M Phase Cell Cycle Checkpoints
Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Cycle::Cell Division::Cell Nucleus Division::Meiosis
Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Cycle::Cell Division::Cell Nucleus Division::Metaphase
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice
Medical Subject Headings::Anatomy::Cells::Cellular Structures::Intracellular Space::Cytoplasm::Cytoplasmic Structures::Cytoskeleton::Microtubule-Organizing Center
Medical Subject Headings::Anatomy::Cells::Cellular Structures::Intracellular Space::Cytoplasm::Cytoplasmic Structures::Cytoskeleton::Microtubules
Medical Subject Headings::Anatomy::Cells::Germ Cells::Ovum::Oocytes
DeCS Terms
CIE Terms
Keywords
Chromosome segregation, Microtubules, Oocytes, Aneuploidy, Metaphase, Spindle poles, Segregación cromosómica, Microtúbulos, Oocitos, Aneuploidia, Metafase, Polos del huso mitótico
Citation
Nabi D, Drechsler H, Pschirer J, Korn F, Schuler N, Diez S, et al. CENP-V is required for proper chromosome segregation through interaction with spindle microtubules in mouse oocytes. Nat Commun. 2021 Nov 11;12(1):6547