RT Journal Article
T1 CENP-V is required for proper chromosome segregation through interaction with spindle microtubules in mouse oocytes
A1 Nabi, Dalileh
A1 Drechsler, Hauke
A1 Pschirer, Johannes
A1 Korn, Franz
A1 Schuler, Nadine
A1 Diez, Stefan
A1 Jessberger, Rolf
A1 Chacón, Mariola
K1 Chromosome segregation
K1 Microtubules
K1 Oocytes
K1 Aneuploidy
K1 Metaphase
K1 Spindle poles
K1 Segregación cromosómica
K1 Microtúbulos
K1 Oocitos
K1 Aneuploidia
K1 Metafase
K1 Polos del huso mitótico
AB Proper chromosome segregation is essential to avoid aneuploidy, yet this process fails with increasing age in mammalian oocytes. Here we report a role for the scarcely described protein CENP-V in oocyte spindle formation and chromosome segregation. We show that depending on the oocyte maturation state, CENP-V localizes to centromeres, to microtubule organizing centers, and to spindle microtubules. We find that Cenp-V-/- oocytes feature severe deficiencies, including metaphase I arrest, strongly reduced polar body extrusion, increased numbers of mis-aligned chromosomes and aneuploidy, multipolar spindles, unfocused spindle poles and loss of kinetochore spindle fibres. We also show that CENP-V protein binds, diffuses along, and bundles microtubules in vitro. The spindle assembly checkpoint arrests about half of metaphase I Cenp-V-/- oocytes from young adults only. This finding suggests checkpoint weakening in ageing oocytes, which mature despite carrying mis-aligned chromosomes. Thus, CENP-V is a microtubule bundling protein crucial to faithful oocyte meiosis, and Cenp-V-/- oocytes reveal age-dependent weakening of the spindle assembly checkpoint.
PB Springer Nature
YR 2021
FD 2021-11-11
LK http://hdl.handle.net/10668/4413
UL http://hdl.handle.net/10668/4413
LA en
NO Nabi D, Drechsler H, Pschirer J, Korn F, Schuler N, Diez S, et al. CENP-V is required for proper chromosome segregation through interaction with spindle microtubules in mouse oocytes. Nat Commun. 2021 Nov 11;12(1):6547
DS RISalud
RD Apr 6, 2025