Publication: A protocol to enrich in undifferentiated cells from neuroblastoma tumor tissue samples and cell lines.
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Identifiers
Date
2022-03-23
Authors
Amador-Alvarez, Aida
Gomez-Muñoz, María A
Rodriguez-Prieto, Ismael
Pardal, Ricardo
Vega, Francisco M
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Journal ISSN
Volume Title
Publisher
Cell Press
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Abstract
The existence of a subpopulation of undifferentiated cells with stem-like properties has been suggested in neuroblastoma tumors, but a definitive biomarker for their successful isolation is missing. Here we describe an in vitro culture system for the enrichment in undifferentiated stem-like tumor cells for subsequent functional assays. We make use of clonal non-adherent cell culture conditions together with cell sorting with specific expression markers. This protocol allows for the differential study of heterogeneous cell population in neuroblastoma tumors. For complete details on the use and execution of this protocol, please refer to Vega et al. (2019).
Description
MeSH Terms
Cell Culture Techniques
Cell Line
Cell Separation
Cells, Cultured
Humans
Neuroblastoma
Cell Line
Cell Separation
Cells, Cultured
Humans
Neuroblastoma
DeCS Terms
Células
Neoplasias
Neuroblastoma
Microscopía electrónica de transmisión de rastreo
Cultura
Biomarcadores
Técnicas In Vitro
Población
Técnicas de cultivo de célula
Neoplasias
Neuroblastoma
Microscopía electrónica de transmisión de rastreo
Cultura
Biomarcadores
Técnicas In Vitro
Población
Técnicas de cultivo de célula
CIE Terms
Keywords
Cancer, Cell Biology, Cell culture, Cell isolation, Flow Cytometry/Mass Cytometry, Single Cell, Stem Cells
Citation
Amador-Álvarez A, Gómez-Muñoz MA, Rodríguez-Prieto I, Pardal R, Vega FM. A protocol to enrich in undifferentiated cells from neuroblastoma tumor tissue samples and cell lines. STAR Protoc. 2022 Mar 23;3(2):101260.