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A protocol to enrich in undifferentiated cells from neuroblastoma tumor tissue samples and cell lines.

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dc.contributor.authorAmador-Alvarez, Aida
dc.contributor.authorGomez-Muñoz, María A
dc.contributor.authorRodriguez-Prieto, Ismael
dc.contributor.authorPardal, Ricardo
dc.contributor.authorVega, Francisco M
dc.contributor.funderJunta de Andalucía-Universidad de Sevilla
dc.contributor.funderMCIN
dc.date.accessioned2023-05-03T15:19:32Z
dc.date.available2023-05-03T15:19:32Z
dc.date.issued2022-03-23
dc.description.abstractThe existence of a subpopulation of undifferentiated cells with stem-like properties has been suggested in neuroblastoma tumors, but a definitive biomarker for their successful isolation is missing. Here we describe an in vitro culture system for the enrichment in undifferentiated stem-like tumor cells for subsequent functional assays. We make use of clonal non-adherent cell culture conditions together with cell sorting with specific expression markers. This protocol allows for the differential study of heterogeneous cell population in neuroblastoma tumors. For complete details on the use and execution of this protocol, please refer to Vega et al. (2019).
dc.description.sponsorshipThis research is supported by grants PID2019-110817RB-I00 funded by MCIN/AEI/ 10.13039/501100011033, grants P18-RT-3151 and US-1262985 funded by Junta de Andalucía-Universidad de Sevilla, and by “ERDF A way of making Europe”. M.A.G. was partially supported by a fellowship from the “Asociación Niños Enfermos de Neuroblastoma (NEN)”. A.A.A. is supported by a FPU grant from the Ministry of Universities.
dc.description.versionSi
dc.identifier.citationAmador-Álvarez A, Gómez-Muñoz MA, Rodríguez-Prieto I, Pardal R, Vega FM. A protocol to enrich in undifferentiated cells from neuroblastoma tumor tissue samples and cell lines. STAR Protoc. 2022 Mar 23;3(2):101260.
dc.identifier.doi10.1016/j.xpro.2022.101260
dc.identifier.essn2666-1667
dc.identifier.pmcPMC8956940
dc.identifier.pmid35345594
dc.identifier.pubmedURLhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8956940/pdf
dc.identifier.unpaywallURLhttps://doi.org/10.1016/j.xpro.2022.101260
dc.identifier.urihttp://hdl.handle.net/10668/22542
dc.issue.number2
dc.journal.titleSTAR protocols
dc.journal.titleabbreviationSTAR Protoc
dc.language.isoen
dc.organizationInstituto de Biomedicina de Sevilla-IBIS
dc.page.number16
dc.provenanceRealizada la curación de contenido 07/03/2025
dc.publisherCell Press
dc.pubmedtypeJournal Article
dc.pubmedtypeResearch Support, Non-U.S. Gov't
dc.relation.projectID10.13039/501100011033
dc.relation.projectIDP18-RT-3151
dc.relation.projectIDUS-1262985
dc.relation.projectIDPID2019-110817RB-I00
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCancer
dc.subjectCell Biology
dc.subjectCell culture
dc.subjectCell isolation
dc.subjectFlow Cytometry/Mass Cytometry
dc.subjectSingle Cell
dc.subjectStem Cells
dc.subject.decsCélulas
dc.subject.decsNeoplasias
dc.subject.decsNeuroblastoma
dc.subject.decsMicroscopía electrónica de transmisión de rastreo
dc.subject.decsCultura
dc.subject.decsBiomarcadores
dc.subject.decsTécnicas In Vitro
dc.subject.decsPoblación
dc.subject.decsTécnicas de cultivo de célula
dc.subject.meshCell Culture Techniques
dc.subject.meshCell Line
dc.subject.meshCell Separation
dc.subject.meshCells, Cultured
dc.subject.meshHumans
dc.subject.meshNeuroblastoma
dc.titleA protocol to enrich in undifferentiated cells from neuroblastoma tumor tissue samples and cell lines.
dc.typeresearch article
dc.type.hasVersionVoR
dc.volume.number3
dspace.entity.typePublication

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