RT Journal Article
T1 A protocol to enrich in undifferentiated cells from neuroblastoma tumor tissue samples and cell lines.
A1 Amador-Alvarez, Aida
A1 Gomez-Muñoz, María A
A1 Rodriguez-Prieto, Ismael
A1 Pardal, Ricardo
A1 Vega, Francisco M
K1 Cancer
K1 Cell Biology
K1 Cell culture
K1 Cell isolation
K1 Flow Cytometry/Mass Cytometry
K1 Single Cell
K1 Stem Cells
AB The existence of a subpopulation of undifferentiated cells with stem-like properties has been suggested in neuroblastoma tumors, but a definitive biomarker for their successful isolation is missing. Here we describe an in vitro culture system for the enrichment in undifferentiated stem-like tumor cells for subsequent functional assays. We make use of clonal non-adherent cell culture conditions together with cell sorting with specific expression markers. This protocol allows for the differential study of heterogeneous cell population in neuroblastoma tumors. For complete details on the use and execution of this protocol, please refer to Vega et al. (2019).
PB Cell Press
YR 2022
FD 2022-03-23
LK http://hdl.handle.net/10668/22542
UL http://hdl.handle.net/10668/22542
LA en
NO Amador-Álvarez A, Gómez-Muñoz MA, Rodríguez-Prieto I, Pardal R, Vega FM. A protocol to enrich in undifferentiated cells from neuroblastoma tumor tissue samples and cell lines. STAR Protoc. 2022 Mar 23;3(2):101260.
NO This research is supported by grants PID2019-110817RB-I00 funded by MCIN/AEI/ 10.13039/501100011033, grants P18-RT-3151 and US-1262985 funded by Junta de Andalucía-Universidad de Sevilla, and by “ERDF A way of making Europe”. M.A.G. was partially supported by a fellowship from the “Asociación Niños Enfermos de Neuroblastoma (NEN)”. A.A.A. is supported by a FPU grant from the Ministry of Universities.
DS RISalud
RD Apr 12, 2025