Publication: Influence of reduction in the elution times on HPLC glycohaemoglobin results
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Identifiers
Date
2009-07-16
Authors
Leon-Justel, Antonio
Santotoribio, Jose D.
Dominguez-Pascual, Inmaculada
Delgado, Ana L.
Macias, Carlos
Herrera, Maria T.
Leal, Alfonso
Mangas, Miguel A.
Acosta, Domingo
Guerrero, J.M.
Advisors
Journal Title
Journal ISSN
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Publisher
Elsevier
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Abstract
Objectives
To compare HPLC methods with short and long elution times for HbA1c measurement in blood.
Methods
Comparison between G7-Tosoh (1.2 min); Bio-Rad-Variant-II-Turbo (1.3 min) and Arkray-HA-8160 (2.9 min).
Results
Passing–Bablok regression equations were: Y = 0.17 + 0.96X; Y = − 0.39 + 1.01X; Y = − 0.40 + 1.0X and the means of the differences using Bland–Altman Plot were 0.02; − 0.34; 0.32 for HA-8160/G7-Tosoh, HA-8160/Variant-II-Turbo and G7-Tosoh/Variant-II-Turbo, respectively.
Conclusions
Faster elution methods had no problems on reproducibility of results obtained by slower elution methods.
Description
MeSH Terms
DeCS Terms
Cromatografía líquida de alta presión
Sangre
Sangre
CIE Terms
Keywords
Cromatografía líquida de alta presión, Sangre
Citation
Leon-Justel A, Santotoribio JD, Dominguez-Pascual I, Delgado AL, Macias C, Herrera MT, et al. Influence of reduction in the elution times on HPLC glycohaemoglobin results. Clin Biochem. 2009 Oct;42(15):1582-4.