RT Journal Article
T1 Influence of reduction in the elution times on HPLC glycohaemoglobin results
A1 Leon-Justel, Antonio
A1 Santotoribio, Jose D.
A1 Dominguez-Pascual, Inmaculada
A1 Delgado, Ana L.
A1 Macias, Carlos
A1 Herrera, Maria T.
A1 Leal, Alfonso
A1 Mangas, Miguel A.
A1 Acosta, Domingo
A1 Guerrero, J.M.
K1 Cromatografía líquida de alta presión
K1 Sangre
AB ObjectivesTo compare HPLC methods with short and long elution times for HbA1c measurement in blood.MethodsComparison between G7-Tosoh (1.2 min); Bio-Rad-Variant-II-Turbo (1.3 min) and Arkray-HA-8160 (2.9 min).ResultsPassing–Bablok regression equations were: Y = 0.17 + 0.96X; Y = − 0.39 + 1.01X; Y = − 0.40 + 1.0X and the means of the differences using Bland–Altman Plot were 0.02; − 0.34; 0.32 for HA-8160/G7-Tosoh, HA-8160/Variant-II-Turbo and G7-Tosoh/Variant-II-Turbo, respectively.ConclusionsFaster elution methods had no problems on reproducibility of results obtained by slower elution methods.
PB Elsevier
SN 1873-2933
YR 2009
FD 2009-07-16
LK https://hdl.handle.net/10668/23207
UL https://hdl.handle.net/10668/23207
LA en
NO Leon-Justel A, Santotoribio JD, Dominguez-Pascual I, Delgado AL, Macias C, Herrera MT, et al. Influence of reduction in the elution times on HPLC glycohaemoglobin results. Clin Biochem. 2009 Oct;42(15):1582-4.
DS RISalud
RD Apr 12, 2025