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Influence of reduction in the elution times on HPLC glycohaemoglobin results

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dc.contributor.authorLeon-Justel, Antonio
dc.contributor.authorSantotoribio, Jose D.
dc.contributor.authorDominguez-Pascual, Inmaculada
dc.contributor.authorDelgado, Ana L.
dc.contributor.authorMacias, Carlos
dc.contributor.authorHerrera, Maria T.
dc.contributor.authorLeal, Alfonso
dc.contributor.authorMangas, Miguel A.
dc.contributor.authorAcosta, Domingo
dc.contributor.authorGuerrero, J.M.
dc.contributor.authoraffiliation[Leon-Justel,A; Santotoribio,JD; Dominguez-Pascual,I; Delgado,AL; Macias,C; Herrera,MT; Guerrero,JM] Department of Clinical Biochemistry, Virgen del Rocío University Hospital, IBIS/CSIC/University of Seville, Seville, Spain.
dc.contributor.authoraffiliation[Leal,A; Mangas,MA; Acosta,D] Clinical Management Unit for Endocrinology and Nutrition, Virgen del Rocío University Hospital, IBIS/CSIC/University of Seville, Seville, Spain.
dc.date.accessioned2024-01-30T14:14:33Z
dc.date.available2024-01-30T14:14:33Z
dc.date.issued2009-07-16
dc.description.abstractObjectives To compare HPLC methods with short and long elution times for HbA1c measurement in blood. Methods Comparison between G7-Tosoh (1.2 min); Bio-Rad-Variant-II-Turbo (1.3 min) and Arkray-HA-8160 (2.9 min). Results Passing–Bablok regression equations were: Y = 0.17 + 0.96X; Y = − 0.39 + 1.01X; Y = − 0.40 + 1.0X and the means of the differences using Bland–Altman Plot were 0.02; − 0.34; 0.32 for HA-8160/G7-Tosoh, HA-8160/Variant-II-Turbo and G7-Tosoh/Variant-II-Turbo, respectively. Conclusions Faster elution methods had no problems on reproducibility of results obtained by slower elution methods.
dc.description.versionYes
dc.identifier.citationLeon-Justel A, Santotoribio JD, Dominguez-Pascual I, Delgado AL, Macias C, Herrera MT, et al. Influence of reduction in the elution times on HPLC glycohaemoglobin results. Clin Biochem. 2009 Oct;42(15):1582-4.
dc.identifier.doi10.1016/j.clinbiochem.2009.07.003
dc.identifier.issn1873-2933
dc.identifier.pmid19615990
dc.identifier.urihttps://hdl.handle.net/10668/23207
dc.issue.number15
dc.journal.titleClinical Biochemistry
dc.language.isoen
dc.page.number3 p.
dc.publisherElsevier
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0009912009002963?via%3Dihub
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsrestricted access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCromatografía líquida de alta presión
dc.subjectSangre
dc.subject.decsCromatografía líquida de alta presión
dc.subject.decsSangre
dc.titleInfluence of reduction in the elution times on HPLC glycohaemoglobin results
dc.typeresearch article
dc.volume.number42
dspace.entity.typePublication

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