Publication: Derivation of HVR1, HVR2 and HVR3 human embryonic stem cell lines from IVF embryos after preimplantation genetic diagnosis (PGD) for monogenic disorder.
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Identifiers
Date
2016-05
Authors
Hmadcha, Abdelkrim
Aguilera, Yolanda
Lozano-Arana, Maria Dolores
Mellado, Nuria
Sánchez, Javier
Moya, Cristina
Sánchez-Palazón, Luis
Palacios, Jose
Antiñolo, Guillermo
Soria, Bernat
Advisors
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
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Abstract
From 106 human blastocyts donate for research after in vitro fertilization (IVF) and preimplantation genetic diagnosis (PGD) for monogenetic disorder, 3 human embryonic stem cells (hESCs) HVR1, HVR2 and HVR3 were successfully derived. HVR1 was assumed to be genetically normal, HVR2 carrying Becker muscular dystrophy and HVR3 Hemophilia B. Despite the translocation t(9;15)(q34.3;q14) detected in HVR2, all the 3 cell lines were characterised in vitro and in vivo as normal hESCs lines and were registered in the Spanish Stem Cell Bank.
Description
Lab resource
MeSH Terms
Medical Subject Headings::Anatomy::Cells::Stem Cells::Embryonic Stem Cells::Human Embryonic Stem Cells
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Reproductive Techniques::Reproductive Techniques, Assisted::Fertilization in Vitro
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Diagnostic Techniques, Obstetrical and Gynecological::Preimplantation Diagnosis
Medical Subject Headings::Anatomy::Cells::Cells, Cultured::Cell Line
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Embryo Research
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Reproductive Techniques::Reproductive Techniques, Assisted::Fertilization in Vitro
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Diagnostic Techniques, Obstetrical and Gynecological::Preimplantation Diagnosis
Medical Subject Headings::Anatomy::Cells::Cells, Cultured::Cell Line
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Embryo Research
DeCS Terms
CIE Terms
Keywords
Células madre embrionarias, Fertilización in Vitro, Investigación básica, Investigaciones con embriones
Citation
Hmadcha A, Aguilera Y, Lozano-Arana MD, Mellado N, Sánchez J, Moya C, et al. Derivation of HVR1, HVR2 and HVR3 human embryonic stem cell lines from IVF embryos after preimplantation genetic diagnosis (PGD) for monogenic disorder. Stem Cell Res. 2016;16(3):635-9