Publication: Assay for high glucose-mediated islet cell sensitization to apoptosis induced by streptozotocin and cytokines
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Date
2005
Authors
Mellado-Gil, Jose M
Aguilar-Diosdado, Manuel
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Publisher
Springer
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Abstract
Pancreatic beta-cell apoptosis is known to participate in the beta-cell destruction process that occurs in diabetes. It has been described that high glucose level induces a hyperfunctional status which could provoke apoptosis. This phenomenon is known as glucotoxicity and has been proposed that it can play a role in type 1 diabetes mellitus pathogenesis. In this study we develop an experimental design to sensitize pancreatic islet cells by high glucose to streptozotocin (STZ) and proinflammatory cytokines [interleukin (IL)-1beta, tumor necrosis factor (TNF)-alpha and interferon (IFN)-gamma]-induced apoptosis. This method is appropriate for subsequent quantification of apoptotic islet cells stained with Tdt-mediated dUTP Nick-End Labeling (TUNEL) and protein expression assays by Western Blotting (WB).
Description
Journal Article;
MeSH Terms
Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Death::Apoptosis
Medical Subject Headings::Diseases::Nutritional and Metabolic Diseases::Metabolic Diseases::Glucose Metabolism Disorders::Diabetes Mellitus
Medical Subject Headings::Chemicals and Drugs::Organic Chemicals::Nitroso Compounds::Nitrosourea Compounds::Streptozocin
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Intercellular Signaling Peptides and Proteins::Cytokines
Medical Subject Headings::Diseases::Nutritional and Metabolic Diseases::Metabolic Diseases::Glucose Metabolism Disorders::Diabetes Mellitus
Medical Subject Headings::Chemicals and Drugs::Organic Chemicals::Nitroso Compounds::Nitrosourea Compounds::Streptozocin
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Intercellular Signaling Peptides and Proteins::Cytokines
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Keywords
Apoptosis, Diabetes Mellitus, Estreptozotocina, Citocinas
Citation
Mellado-Gil JM, Aguilar-Diosdado M. Assay for high glucose-mediated islet cell sensitization to apoptosis induced by streptozotocin and cytokines. Biol Proced Online; 2005, 7:162-71