%0 Journal Article
%A Mellado-Gil, Jose M
%A Aguilar-Diosdado, Manuel
%T Assay for high glucose-mediated islet cell sensitization to apoptosis induced by streptozotocin and cytokines
%D 2005
%@ 1480-9222
%U http://hdl.handle.net/10668/581
%X Pancreatic beta-cell apoptosis is known to participate in the beta-cell destruction process that occurs in diabetes. It has been described that high glucose level induces a hyperfunctional status which could provoke apoptosis. This phenomenon is known as glucotoxicity and has been proposed that it can play a role in type 1 diabetes mellitus pathogenesis. In this study we develop an experimental design to sensitize pancreatic islet cells by high glucose to streptozotocin (STZ) and proinflammatory cytokines [interleukin (IL)-1beta, tumor necrosis factor (TNF)-alpha and interferon (IFN)-gamma]-induced apoptosis. This method is appropriate for subsequent quantification of apoptotic islet cells stained with Tdt-mediated dUTP Nick-End Labeling (TUNEL) and protein expression assays by Western Blotting (WB).
%K Apoptosis
%K Diabetes Mellitus
%K Estreptozotocina
%K Citocinas
%~