RT Journal Article
T1 Assay for high glucose-mediated islet cell sensitization to apoptosis induced by streptozotocin and cytokines
A1 Mellado-Gil, Jose M
A1 Aguilar-Diosdado, Manuel
K1 Apoptosis
K1 Diabetes Mellitus
K1 Estreptozotocina
K1 Citocinas
AB Pancreatic beta-cell apoptosis is known to participate in the beta-cell destruction process that occurs in diabetes. It has been described that high glucose level induces a hyperfunctional status which could provoke apoptosis. This phenomenon is known as glucotoxicity and has been proposed that it can play a role in type 1 diabetes mellitus pathogenesis. In this study we develop an experimental design to sensitize pancreatic islet cells by high glucose to streptozotocin (STZ) and proinflammatory cytokines [interleukin (IL)-1beta, tumor necrosis factor (TNF)-alpha and interferon (IFN)-gamma]-induced apoptosis. This method is appropriate for subsequent quantification of apoptotic islet cells stained with Tdt-mediated dUTP Nick-End Labeling (TUNEL) and protein expression assays by Western Blotting (WB).
PB Springer
SN 1480-9222
YR 2005
FD 2005
LK http://hdl.handle.net/10668/581
UL http://hdl.handle.net/10668/581
LA en
NO Mellado-Gil JM, Aguilar-Diosdado M. Assay for high glucose-mediated islet cell sensitization to apoptosis induced by streptozotocin and cytokines. Biol Proced Online; 2005, 7:162-71
NO Journal Article;
DS RISalud
RD Apr 8, 2025