Publication: Selective accumulation of biotin in arterial chemoreceptors: requirement for carotid body exocytotic dopamine secretion
No Thumbnail Available
Identifiers
Date
2016-12-14
Authors
Ortega-Saenz, Patricia
Macias, David
Levitsky, Konstantin L.
Rodriguez-Gomez, Jose A.
Gonzalez-Rodriguez, Patricia
Bonilla-Henao, Victoria
Arias-Mayenco, Ignacio
Lopez-Barneo, Jose
Advisors
Journal Title
Journal ISSN
Volume Title
Publisher
Wiley-blackwell
Abstract
Biotin is a water-soluble vitamin required for the function of carboxylases as well as for the regulation of gene expression. Here, we report that biotin accumulates in unusually large amounts in cells of arterial chemoreceptors, carotid body (CB) and adrenal medulla (AM). We show in a biotin-deficient rat model that the vitamin rapidly disappears from the blood and other tissues (including the AM), while remaining at relatively high levels in the CB. We have also observed that, in comparison with other peripheral neural tissues, CB cells contain high levels of SLC5a6, a biotin transporter, and SLC19a3, a thiamine transporter regulated by biotin. Biotin-deficient rats show a syndrome characterized by marked weight loss, metabolic lactic acidosis, aciduria and accelerated breathing with normal responsiveness to hypoxia. Remarkably, CB cells from biotin-deficient animals have normal electrophysiological and neurochemical (ATP levels and catecholamine synthesis) properties; however, they exhibit a marked decrease in the size of quantal catecholaminergic secretory events, which is not seen in AM cells. A similar differential secretory dysfunction is observed in CB cells treated with tetrabenazine, a selective inhibitor of the vesicular monoamine transporter 2 (VMAT2). VMAT2 is highly expressed in glomus cells (in comparison with VMAT1), and in biotin-deficient animals VMAT2 protein expression decreases in parallel with the decrease of biotin accumulated in CB cells. These data suggest that biotin has an essential role in the homeostasis of dopaminergic transmission modulating the transport and/or storage of transmitters within small secretory granules in glomus cells.
Description
MeSH Terms
Vesicular Monoamine Transport Proteins
Carotid Body
biotin transporter
Tetrabenazine
Homeostasis
Carotid Body
biotin transporter
Tetrabenazine
Homeostasis
DeCS Terms
Inmunohistoquímica
Biotina
Ratas Sprague-Dawley
Proliferación celular
Tetrabenazina
Proteínas de transporte vesicular de monoaminas
Biotina
Ratas Sprague-Dawley
Proliferación celular
Tetrabenazina
Proteínas de transporte vesicular de monoaminas
CIE Terms
Keywords
arterial chemoreceptors, biotin, carotid body, Basal ganglia disease, Multiple carboxylase deficiency, Blood-brain-barrier, Glomus cells, Asialoglycoprotein receptor, Transporter family, Chromaffin cells, Hypoxia, Mechanisms, Inhibition
Citation
Ortega-Sáenz P, Macías D, Levitsky KL, Rodríguez-Gómez JA, González-Rodríguez P, Bonilla-Henao V, et al. Selective accumulation of biotin in arterial chemoreceptors: requirement for carotid body exocytotic dopamine secretion. J Physiol. 2016 Dec 15;594(24):7229-7248.