Publication: Selective accumulation of biotin in arterial chemoreceptors: requirement for carotid body exocytotic dopamine secretion
dc.contributor.author | Ortega-Saenz, Patricia | |
dc.contributor.author | Macias, David | |
dc.contributor.author | Levitsky, Konstantin L. | |
dc.contributor.author | Rodriguez-Gomez, Jose A. | |
dc.contributor.author | Gonzalez-Rodriguez, Patricia | |
dc.contributor.author | Bonilla-Henao, Victoria | |
dc.contributor.author | Arias-Mayenco, Ignacio | |
dc.contributor.author | Lopez-Barneo, Jose | |
dc.contributor.authoraffiliation | [Ortega-Saenz, Patricia] Univ Seville, CSIC, Hosp Univ Virgen Rocio, Inst Biomed Sevilla IBiS, Seville, Spain | |
dc.contributor.authoraffiliation | [Macias, David] Univ Seville, CSIC, Hosp Univ Virgen Rocio, Inst Biomed Sevilla IBiS, Seville, Spain | |
dc.contributor.authoraffiliation | [Levitsky, Konstantin L.] Univ Seville, CSIC, Hosp Univ Virgen Rocio, Inst Biomed Sevilla IBiS, Seville, Spain | |
dc.contributor.authoraffiliation | [Rodriguez-Gomez, Jose A.] Univ Seville, CSIC, Hosp Univ Virgen Rocio, Inst Biomed Sevilla IBiS, Seville, Spain | |
dc.contributor.authoraffiliation | [Gonzalez-Rodriguez, Patricia] Univ Seville, CSIC, Hosp Univ Virgen Rocio, Inst Biomed Sevilla IBiS, Seville, Spain | |
dc.contributor.authoraffiliation | [Bonilla-Henao, Victoria] Univ Seville, CSIC, Hosp Univ Virgen Rocio, Inst Biomed Sevilla IBiS, Seville, Spain | |
dc.contributor.authoraffiliation | [Arias-Mayenco, Ignacio] Univ Seville, CSIC, Hosp Univ Virgen Rocio, Inst Biomed Sevilla IBiS, Seville, Spain | |
dc.contributor.authoraffiliation | [Lopez-Barneo, Jose] Univ Seville, CSIC, Hosp Univ Virgen Rocio, Inst Biomed Sevilla IBiS, Seville, Spain | |
dc.contributor.authoraffiliation | [Ortega-Saenz, Patricia] Univ Seville, Fac Med, Dept Fisiol Med & Biofis, Seville, Spain | |
dc.contributor.authoraffiliation | [Rodriguez-Gomez, Jose A.] Univ Seville, Fac Med, Dept Fisiol Med & Biofis, Seville, Spain | |
dc.contributor.authoraffiliation | [Gonzalez-Rodriguez, Patricia] Univ Seville, Fac Med, Dept Fisiol Med & Biofis, Seville, Spain | |
dc.contributor.authoraffiliation | [Bonilla-Henao, Victoria] Univ Seville, Fac Med, Dept Fisiol Med & Biofis, Seville, Spain | |
dc.contributor.authoraffiliation | [Arias-Mayenco, Ignacio] Univ Seville, Fac Med, Dept Fisiol Med & Biofis, Seville, Spain | |
dc.contributor.authoraffiliation | [Lopez-Barneo, Jose] Univ Seville, Fac Med, Dept Fisiol Med & Biofis, Seville, Spain | |
dc.contributor.authoraffiliation | [Ortega-Saenz, Patricia] Ctr Invest Biomed Red Enfermedades Neurodegenerat, Madrid, Spain | |
dc.contributor.authoraffiliation | [Gonzalez-Rodriguez, Patricia] Ctr Invest Biomed Red Enfermedades Neurodegenerat, Madrid, Spain | |
dc.contributor.authoraffiliation | [Bonilla-Henao, Victoria] Ctr Invest Biomed Red Enfermedades Neurodegenerat, Madrid, Spain | |
dc.contributor.authoraffiliation | [Arias-Mayenco, Ignacio] Ctr Invest Biomed Red Enfermedades Neurodegenerat, Madrid, Spain | |
dc.contributor.authoraffiliation | [Lopez-Barneo, Jose] Ctr Invest Biomed Red Enfermedades Neurodegenerat, Madrid, Spain | |
dc.contributor.authoraffiliation | [Macias, David] Univ Cambridge, Dept Physiol Dev & Neurosci, Cambridge CB2 1TN, England | |
dc.contributor.funder | Botin Foundation | |
dc.contributor.funder | Spanish Ministries of Health and of Economy and Competitiveness | |
dc.contributor.funder | European Research Council (ERC) | |
dc.date.accessioned | 2023-02-12T02:21:35Z | |
dc.date.available | 2023-02-12T02:21:35Z | |
dc.date.issued | 2016-12-14 | |
dc.description.abstract | Biotin is a water-soluble vitamin required for the function of carboxylases as well as for the regulation of gene expression. Here, we report that biotin accumulates in unusually large amounts in cells of arterial chemoreceptors, carotid body (CB) and adrenal medulla (AM). We show in a biotin-deficient rat model that the vitamin rapidly disappears from the blood and other tissues (including the AM), while remaining at relatively high levels in the CB. We have also observed that, in comparison with other peripheral neural tissues, CB cells contain high levels of SLC5a6, a biotin transporter, and SLC19a3, a thiamine transporter regulated by biotin. Biotin-deficient rats show a syndrome characterized by marked weight loss, metabolic lactic acidosis, aciduria and accelerated breathing with normal responsiveness to hypoxia. Remarkably, CB cells from biotin-deficient animals have normal electrophysiological and neurochemical (ATP levels and catecholamine synthesis) properties; however, they exhibit a marked decrease in the size of quantal catecholaminergic secretory events, which is not seen in AM cells. A similar differential secretory dysfunction is observed in CB cells treated with tetrabenazine, a selective inhibitor of the vesicular monoamine transporter 2 (VMAT2). VMAT2 is highly expressed in glomus cells (in comparison with VMAT1), and in biotin-deficient animals VMAT2 protein expression decreases in parallel with the decrease of biotin accumulated in CB cells. These data suggest that biotin has an essential role in the homeostasis of dopaminergic transmission modulating the transport and/or storage of transmitters within small secretory granules in glomus cells. | |
dc.description.version | Si | |
dc.identifier.citation | Ortega-Sáenz P, Macías D, Levitsky KL, Rodríguez-Gómez JA, González-Rodríguez P, Bonilla-Henao V, et al. Selective accumulation of biotin in arterial chemoreceptors: requirement for carotid body exocytotic dopamine secretion. J Physiol. 2016 Dec 15;594(24):7229-7248. | |
dc.identifier.doi | 10.1113/JP272961 | |
dc.identifier.essn | 1469-7793 | |
dc.identifier.issn | 0022-3751 | |
dc.identifier.unpaywallURL | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5157066 | |
dc.identifier.uri | http://hdl.handle.net/10668/18996 | |
dc.identifier.wosID | 390652800019 | |
dc.issue.number | 24 | |
dc.journal.title | Journal of physiology-london | |
dc.journal.titleabbreviation | J. physiol.-london | |
dc.language.iso | en | |
dc.organization | Instituto de Biomedicina de Sevilla-IBIS | |
dc.organization | Hospital Universitario Virgen del Rocío | |
dc.page.number | 7229-7248 | |
dc.provenance | Realizada la curación de contenido 11/07/2025. | |
dc.publisher | Wiley-blackwell | |
dc.relation.projectID | SAF2012-39343 | |
dc.relation.projectID | PIE13/0004 | |
dc.relation.projectID | ERC:PRJ201502629 | |
dc.relation.publisherversion | https://doi.org/10.1113/JP272961 | |
dc.rights.accessRights | Restricted Access | |
dc.subject | arterial chemoreceptors | |
dc.subject | biotin | |
dc.subject | carotid body | |
dc.subject | Basal ganglia disease | |
dc.subject | Multiple carboxylase deficiency | |
dc.subject | Blood-brain-barrier | |
dc.subject | Glomus cells | |
dc.subject | Asialoglycoprotein receptor | |
dc.subject | Transporter family | |
dc.subject | Chromaffin cells | |
dc.subject | Hypoxia | |
dc.subject | Mechanisms | |
dc.subject | Inhibition | |
dc.subject.decs | Inmunohistoquímica | |
dc.subject.decs | Biotina | |
dc.subject.decs | Ratas Sprague-Dawley | |
dc.subject.decs | Proliferación celular | |
dc.subject.decs | Tetrabenazina | |
dc.subject.decs | Proteínas de transporte vesicular de monoaminas | |
dc.subject.mesh | Vesicular Monoamine Transport Proteins | |
dc.subject.mesh | Carotid Body | |
dc.subject.mesh | biotin transporter | |
dc.subject.mesh | Tetrabenazine | |
dc.subject.mesh | Homeostasis | |
dc.title | Selective accumulation of biotin in arterial chemoreceptors: requirement for carotid body exocytotic dopamine secretion | |
dc.type | research article | |
dc.type.hasVersion | VoR | |
dc.volume.number | 594 | |
dc.wostype | Article | |
dspace.entity.type | Publication |
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