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Selective accumulation of biotin in arterial chemoreceptors: requirement for carotid body exocytotic dopamine secretion

dc.contributor.authorOrtega-Saenz, Patricia
dc.contributor.authorMacias, David
dc.contributor.authorLevitsky, Konstantin L.
dc.contributor.authorRodriguez-Gomez, Jose A.
dc.contributor.authorGonzalez-Rodriguez, Patricia
dc.contributor.authorBonilla-Henao, Victoria
dc.contributor.authorArias-Mayenco, Ignacio
dc.contributor.authorLopez-Barneo, Jose
dc.contributor.authoraffiliation[Ortega-Saenz, Patricia] Univ Seville, CSIC, Hosp Univ Virgen Rocio, Inst Biomed Sevilla IBiS, Seville, Spain
dc.contributor.authoraffiliation[Macias, David] Univ Seville, CSIC, Hosp Univ Virgen Rocio, Inst Biomed Sevilla IBiS, Seville, Spain
dc.contributor.authoraffiliation[Levitsky, Konstantin L.] Univ Seville, CSIC, Hosp Univ Virgen Rocio, Inst Biomed Sevilla IBiS, Seville, Spain
dc.contributor.authoraffiliation[Rodriguez-Gomez, Jose A.] Univ Seville, CSIC, Hosp Univ Virgen Rocio, Inst Biomed Sevilla IBiS, Seville, Spain
dc.contributor.authoraffiliation[Gonzalez-Rodriguez, Patricia] Univ Seville, CSIC, Hosp Univ Virgen Rocio, Inst Biomed Sevilla IBiS, Seville, Spain
dc.contributor.authoraffiliation[Bonilla-Henao, Victoria] Univ Seville, CSIC, Hosp Univ Virgen Rocio, Inst Biomed Sevilla IBiS, Seville, Spain
dc.contributor.authoraffiliation[Arias-Mayenco, Ignacio] Univ Seville, CSIC, Hosp Univ Virgen Rocio, Inst Biomed Sevilla IBiS, Seville, Spain
dc.contributor.authoraffiliation[Lopez-Barneo, Jose] Univ Seville, CSIC, Hosp Univ Virgen Rocio, Inst Biomed Sevilla IBiS, Seville, Spain
dc.contributor.authoraffiliation[Ortega-Saenz, Patricia] Univ Seville, Fac Med, Dept Fisiol Med & Biofis, Seville, Spain
dc.contributor.authoraffiliation[Rodriguez-Gomez, Jose A.] Univ Seville, Fac Med, Dept Fisiol Med & Biofis, Seville, Spain
dc.contributor.authoraffiliation[Gonzalez-Rodriguez, Patricia] Univ Seville, Fac Med, Dept Fisiol Med & Biofis, Seville, Spain
dc.contributor.authoraffiliation[Bonilla-Henao, Victoria] Univ Seville, Fac Med, Dept Fisiol Med & Biofis, Seville, Spain
dc.contributor.authoraffiliation[Arias-Mayenco, Ignacio] Univ Seville, Fac Med, Dept Fisiol Med & Biofis, Seville, Spain
dc.contributor.authoraffiliation[Lopez-Barneo, Jose] Univ Seville, Fac Med, Dept Fisiol Med & Biofis, Seville, Spain
dc.contributor.authoraffiliation[Ortega-Saenz, Patricia] Ctr Invest Biomed Red Enfermedades Neurodegenerat, Madrid, Spain
dc.contributor.authoraffiliation[Gonzalez-Rodriguez, Patricia] Ctr Invest Biomed Red Enfermedades Neurodegenerat, Madrid, Spain
dc.contributor.authoraffiliation[Bonilla-Henao, Victoria] Ctr Invest Biomed Red Enfermedades Neurodegenerat, Madrid, Spain
dc.contributor.authoraffiliation[Arias-Mayenco, Ignacio] Ctr Invest Biomed Red Enfermedades Neurodegenerat, Madrid, Spain
dc.contributor.authoraffiliation[Lopez-Barneo, Jose] Ctr Invest Biomed Red Enfermedades Neurodegenerat, Madrid, Spain
dc.contributor.authoraffiliation[Macias, David] Univ Cambridge, Dept Physiol Dev & Neurosci, Cambridge CB2 1TN, England
dc.contributor.funderBotin Foundation
dc.contributor.funderSpanish Ministries of Health and of Economy and Competitiveness
dc.contributor.funderEuropean Research Council (ERC)
dc.date.accessioned2023-02-12T02:21:35Z
dc.date.available2023-02-12T02:21:35Z
dc.date.issued2016-12-14
dc.description.abstractBiotin is a water-soluble vitamin required for the function of carboxylases as well as for the regulation of gene expression. Here, we report that biotin accumulates in unusually large amounts in cells of arterial chemoreceptors, carotid body (CB) and adrenal medulla (AM). We show in a biotin-deficient rat model that the vitamin rapidly disappears from the blood and other tissues (including the AM), while remaining at relatively high levels in the CB. We have also observed that, in comparison with other peripheral neural tissues, CB cells contain high levels of SLC5a6, a biotin transporter, and SLC19a3, a thiamine transporter regulated by biotin. Biotin-deficient rats show a syndrome characterized by marked weight loss, metabolic lactic acidosis, aciduria and accelerated breathing with normal responsiveness to hypoxia. Remarkably, CB cells from biotin-deficient animals have normal electrophysiological and neurochemical (ATP levels and catecholamine synthesis) properties; however, they exhibit a marked decrease in the size of quantal catecholaminergic secretory events, which is not seen in AM cells. A similar differential secretory dysfunction is observed in CB cells treated with tetrabenazine, a selective inhibitor of the vesicular monoamine transporter 2 (VMAT2). VMAT2 is highly expressed in glomus cells (in comparison with VMAT1), and in biotin-deficient animals VMAT2 protein expression decreases in parallel with the decrease of biotin accumulated in CB cells. These data suggest that biotin has an essential role in the homeostasis of dopaminergic transmission modulating the transport and/or storage of transmitters within small secretory granules in glomus cells.
dc.description.versionSi
dc.identifier.citationOrtega-Sáenz P, Macías D, Levitsky KL, Rodríguez-Gómez JA, González-Rodríguez P, Bonilla-Henao V, et al. Selective accumulation of biotin in arterial chemoreceptors: requirement for carotid body exocytotic dopamine secretion. J Physiol. 2016 Dec 15;594(24):7229-7248.
dc.identifier.doi10.1113/JP272961
dc.identifier.essn1469-7793
dc.identifier.issn0022-3751
dc.identifier.unpaywallURLhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5157066
dc.identifier.urihttp://hdl.handle.net/10668/18996
dc.identifier.wosID390652800019
dc.issue.number24
dc.journal.titleJournal of physiology-london
dc.journal.titleabbreviationJ. physiol.-london
dc.language.isoen
dc.organizationInstituto de Biomedicina de Sevilla-IBIS
dc.organizationHospital Universitario Virgen del Rocío
dc.page.number7229-7248
dc.provenanceRealizada la curación de contenido 11/07/2025.
dc.publisherWiley-blackwell
dc.relation.projectIDSAF2012-39343
dc.relation.projectIDPIE13/0004
dc.relation.projectIDERC:PRJ201502629
dc.relation.publisherversionhttps://doi.org/10.1113/JP272961
dc.rights.accessRightsRestricted Access
dc.subjectarterial chemoreceptors
dc.subjectbiotin
dc.subjectcarotid body
dc.subjectBasal ganglia disease
dc.subjectMultiple carboxylase deficiency
dc.subjectBlood-brain-barrier
dc.subjectGlomus cells
dc.subjectAsialoglycoprotein receptor
dc.subjectTransporter family
dc.subjectChromaffin cells
dc.subjectHypoxia
dc.subjectMechanisms
dc.subjectInhibition
dc.subject.decsInmunohistoquímica
dc.subject.decsBiotina
dc.subject.decsRatas Sprague-Dawley
dc.subject.decsProliferación celular
dc.subject.decsTetrabenazina
dc.subject.decsProteínas de transporte vesicular de monoaminas
dc.subject.meshVesicular Monoamine Transport Proteins
dc.subject.meshCarotid Body
dc.subject.meshbiotin transporter
dc.subject.meshTetrabenazine
dc.subject.meshHomeostasis
dc.titleSelective accumulation of biotin in arterial chemoreceptors: requirement for carotid body exocytotic dopamine secretion
dc.typeresearch article
dc.type.hasVersionVoR
dc.volume.number594
dc.wostypeArticle
dspace.entity.typePublication

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