Publication: Hoxa9 and EGFP reporter expression in human Embryonic Stem Cells (hESC) as useful tools for studying human development.
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2017-08-05
Authors
Domingo-Reines, Joan
López-Ornelas, Adolfo
Montes, Rosa
Romero, Tamara
Rodriguez-Llamas, Jose Luis
Lara-Rodarte, Rolando
González-Pozas, Federico
Ayllon, Veronica
Menendez, Pablo
Velasco, Iván
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Abstract
HoxA9 is an evolutionarily conserved homeobox gene implicated in embryo development. To study the roles of Hoxa9 during human development we generated a transgenic H9 (hESC) line that overexpresses HoxA9 and the Enhanced Green Fluorescent Protein (EGFP), and a control H9 with a stable expression of the EGFP. The resulting H9-HoxA9-EGFP and H9-EGFP cell lines allow an efficient visualization of hESCs by fluorescent microscopy, quantification by flow cytometry and cell differentiation tracking. Both transgenic cell lines maintained the pluripotent phenotype, the ability to differentiate into all three germ layers and a normal karyotype.
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MeSH Terms
Cell Differentiation
Cells, Cultured
Embryo, Mammalian
Embryonic Development
Embryonic Stem Cells
Female
Gene Expression Regulation, Developmental
Genes, Reporter
Green Fluorescent Proteins
Homeodomain Proteins
Humans
Transfection
Cells, Cultured
Embryo, Mammalian
Embryonic Development
Embryonic Stem Cells
Female
Gene Expression Regulation, Developmental
Genes, Reporter
Green Fluorescent Proteins
Homeodomain Proteins
Humans
Transfection