Publication:
Hoxa9 and EGFP reporter expression in human Embryonic Stem Cells (hESC) as useful tools for studying human development.

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dc.contributor.authorDomingo-Reines, Joan
dc.contributor.authorLópez-Ornelas, Adolfo
dc.contributor.authorMontes, Rosa
dc.contributor.authorRomero, Tamara
dc.contributor.authorRodriguez-Llamas, Jose Luis
dc.contributor.authorLara-Rodarte, Rolando
dc.contributor.authorGonzález-Pozas, Federico
dc.contributor.authorAyllon, Veronica
dc.contributor.authorMenendez, Pablo
dc.contributor.authorVelasco, Iván
dc.contributor.authorRamos-Mejia, Veronica
dc.date.accessioned2023-01-25T10:02:03Z
dc.date.available2023-01-25T10:02:03Z
dc.date.issued2017-08-05
dc.description.abstractHoxA9 is an evolutionarily conserved homeobox gene implicated in embryo development. To study the roles of Hoxa9 during human development we generated a transgenic H9 (hESC) line that overexpresses HoxA9 and the Enhanced Green Fluorescent Protein (EGFP), and a control H9 with a stable expression of the EGFP. The resulting H9-HoxA9-EGFP and H9-EGFP cell lines allow an efficient visualization of hESCs by fluorescent microscopy, quantification by flow cytometry and cell differentiation tracking. Both transgenic cell lines maintained the pluripotent phenotype, the ability to differentiate into all three germ layers and a normal karyotype.
dc.identifier.doi10.1016/j.scr.2017.08.004
dc.identifier.essn1876-7753
dc.identifier.pmid29246576
dc.identifier.unpaywallURLhttps://doi.org/10.1016/j.scr.2017.08.004
dc.identifier.urihttp://hdl.handle.net/10668/11916
dc.journal.titleStem cell research
dc.journal.titleabbreviationStem Cell Res
dc.language.isoen
dc.organizationCentro Pfizer-Universidad de Granada-Junta de Andalucía de Genómica e Investigación Oncológica-GENYO
dc.page.number286-290
dc.pubmedtypeJournal Article
dc.pubmedtypeResearch Support, Non-U.S. Gov't
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.meshCell Differentiation
dc.subject.meshCells, Cultured
dc.subject.meshEmbryo, Mammalian
dc.subject.meshEmbryonic Development
dc.subject.meshEmbryonic Stem Cells
dc.subject.meshFemale
dc.subject.meshGene Expression Regulation, Developmental
dc.subject.meshGenes, Reporter
dc.subject.meshGreen Fluorescent Proteins
dc.subject.meshHomeodomain Proteins
dc.subject.meshHumans
dc.subject.meshTransfection
dc.titleHoxa9 and EGFP reporter expression in human Embryonic Stem Cells (hESC) as useful tools for studying human development.
dc.typeresearch article
dc.type.hasVersionVoR
dc.volume.number25
dspace.entity.typePublication

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