RT Journal Article
T1 Hoxa9 and EGFP reporter expression in human Embryonic Stem Cells (hESC) as useful tools for studying human development.
A1 Domingo-Reines, Joan
A1 López-Ornelas, Adolfo
A1 Montes, Rosa
A1 Romero, Tamara
A1 Rodriguez-Llamas, Jose Luis
A1 Lara-Rodarte, Rolando
A1 González-Pozas, Federico
A1 Ayllon, Veronica
A1 Menendez, Pablo
A1 Velasco, Iván
A1 Ramos-Mejia, Veronica
AB HoxA9 is an evolutionarily conserved homeobox gene implicated in embryo development. To study the roles of Hoxa9 during human development we generated a transgenic H9 (hESC) line that overexpresses HoxA9 and the Enhanced Green Fluorescent Protein (EGFP), and a control H9 with a stable expression of the EGFP. The resulting H9-HoxA9-EGFP and H9-EGFP cell lines allow an efficient visualization of hESCs by fluorescent microscopy, quantification by flow cytometry and cell differentiation tracking. Both transgenic cell lines maintained the pluripotent phenotype, the ability to differentiate into all three germ layers and a normal karyotype.
YR 2017
FD 2017-08-05
LK http://hdl.handle.net/10668/11916
UL http://hdl.handle.net/10668/11916
LA en
DS RISalud
RD Apr 7, 2025