Publication: Importance of tissue sampling, laboratory methods, and patient characteristics for detection of Pneumocystis in autopsied lungs of non-immunosuppressed individuals.
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Identifiers
Date
2017-10
Authors
Vargas, S L
Ponce, C
Bustamante, R
Calderon, E
Nevez, G
De Armas, Y
Matos, O
Miller, R F
Gallo, M J
Advisors
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Journal ISSN
Volume Title
Publisher
Springer
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Abstract
To understand the epidemiological significance of Pneumocystis detection in a lung tissue sample of non-immunosuppressed individuals, we examined sampling procedures, laboratory methodology, and patient characteristics of autopsy series reported in the literature. Number of tissue specimens, DNA-extraction procedures, age and underlying diagnosis highly influence yield and are critical to understand yield differences of Pneumocystis among reports of pulmonary colonization in immunocompetent individuals.
Description
MeSH Terms
Autopsy
Humans
Lung
Microbiological Techniques
Pneumocystis
Pneumonia, Pneumocystis
Specimen Handling
Humans
Lung
Microbiological Techniques
Pneumocystis
Pneumonia, Pneumocystis
Specimen Handling
DeCS Terms
Métodos
Diagnóstico
Pneumocystis
Pulmón
Autopsia
ADN
Diagnóstico
Pneumocystis
Pulmón
Autopsia
ADN
CIE Terms
Keywords
Autopsy Lung, Lung Tissue Weight, Pulmonary Colonization, Jirovecii, Chilled Serum
Citation
Vargas SL, Ponce C, Bustamante R, Calderón E, Nevez G, De Armas Y, et al. Importance of tissue sampling, laboratory methods, and patient characteristics for detection of Pneumocystis in autopsied lungs of non-immunosuppressed individuals. Eur J Clin Microbiol Infect Dis. 2017 Oct;36(10):1711-1716.