RT Generic T1 Importance of tissue sampling, laboratory methods, and patient characteristics for detection of Pneumocystis in autopsied lungs of non-immunosuppressed individuals. A1 Vargas, S L A1 Ponce, C A1 Bustamante, R A1 Calderon, E A1 Nevez, G A1 De Armas, Y A1 Matos, O A1 Miller, R F A1 Gallo, M J K1 Autopsy Lung K1 Lung Tissue Weight K1 Pulmonary Colonization K1 Jirovecii K1 Chilled Serum AB To understand the epidemiological significance of Pneumocystis detection in a lung tissue sample of non-immunosuppressed individuals, we examined sampling procedures, laboratory methodology, and patient characteristics of autopsy series reported in the literature. Number of tissue specimens, DNA-extraction procedures, age and underlying diagnosis highly influence yield and are critical to understand yield differences of Pneumocystis among reports of pulmonary colonization in immunocompetent individuals. PB Springer YR 2017 FD 2017-10 LK http://hdl.handle.net/10668/11275 UL http://hdl.handle.net/10668/11275 LA en NO Vargas SL, Ponce C, Bustamante R, Calderón E, Nevez G, De Armas Y, et al. Importance of tissue sampling, laboratory methods, and patient characteristics for detection of Pneumocystis in autopsied lungs of non-immunosuppressed individuals. Eur J Clin Microbiol Infect Dis. 2017 Oct;36(10):1711-1716. DS RISalud RD Jul 2, 2025