RT Journal Article T1 Importance of tissue sampling, laboratory methods, and patient characteristics for detection of Pneumocystis in autopsied lungs of non-immunosuppressed individuals. A1 Vargas, S L A1 Ponce, C A1 Bustamante, R A1 Calderón, E A1 Nevez, G A1 De Armas, Y A1 Matos, O A1 Miller, R F A1 Gallo, M J AB To understand the epidemiological significance of Pneumocystis detection in a lung tissue sample of non-immunosuppressed individuals, we examined sampling procedures, laboratory methodology, and patient characteristics of autopsy series reported in the literature. Number of tissue specimens, DNA-extraction procedures, age and underlying diagnosis highly influence yield and are critical to understand yield differences of Pneumocystis among reports of pulmonary colonization in immunocompetent individuals. YR 2017 FD 2017-06-05 LK http://hdl.handle.net/10668/11275 UL http://hdl.handle.net/10668/11275 LA en DS RISalud RD Apr 18, 2025