%0 Journal Article %A Vargas, S L %A Ponce, C %A Bustamante, R %A Calderón, E %A Nevez, G %A De Armas, Y %A Matos, O %A Miller, R F %A Gallo, M J %T Importance of tissue sampling, laboratory methods, and patient characteristics for detection of Pneumocystis in autopsied lungs of non-immunosuppressed individuals. %D 2017 %U http://hdl.handle.net/10668/11275 %X To understand the epidemiological significance of Pneumocystis detection in a lung tissue sample of non-immunosuppressed individuals, we examined sampling procedures, laboratory methodology, and patient characteristics of autopsy series reported in the literature. Number of tissue specimens, DNA-extraction procedures, age and underlying diagnosis highly influence yield and are critical to understand yield differences of Pneumocystis among reports of pulmonary colonization in immunocompetent individuals. %~