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Title: A comparative study of the preventative effects exerted by two probiotics, Lactobacillus reuteri and Lactobacillus fermentum, in the trinitrobenzenesulfonic acid model of rat colitis
Authors: Peran, Laura
Sierra, Saleta
Comalada, Mònica
Lara-Villoslada, Federico
Bailón, Elvira
Nieto, Ana
Concha, Angel
Olivares, Mónica
Zarzuelo, Antonio
Xaus, Jordi
Gálvez, Julio
metadata.dc.contributor.authoraffiliation: [Peran,L; Comalada,M; Bailón,E; Zarzuelo,A; Gálvez,J] Department of Pharmacology, University of Granada, Granada, Spain. [Sierra,S; Lara-Villoslada,F; Olivares,M; Xaus,J] Department of Immunology and Animal Sciences, Puleva Biotech SA, Granada, Spain. [Nieto,A] Andalusian Stem Cell Bank, Health and Progress Foundation, Granada, Spain. [Concha,A] Department of Pathology, Hospital Universitario Virgen de las Nieves, Granada, Spain.
Keywords: Probiotics;Inflammatory bowel diseases;Immunomodulation;Anti-inflammatory activity;Colitis;Colon;Diarrea;Ácidos Grasos Volátiles;Heces;Femenino;Contenido Digestivo;Glutatión;Concentración de Iones de Hidrógeno;Enfermedades Inflamatorias del Intestino;Mucosa Intestinal;Lactobacillus fermentum;Lactobacillus reuteri;Modelos Animales;Músculo Esquelético;Tamaño de los Órganos;Peroxidasa;Probióticos;Ratas;Ratas Wistar;Bazo;Timo;Ácido Trinitrobencenosulfónico;Factor de Necrosis Tumoral alfa;Animales
metadata.dc.subject.mesh: Medical Subject Headings::Diseases::Digestive System Diseases::Gastrointestinal Diseases::Gastroenteritis::Colitis
Medical Subject Headings::Anatomy::Digestive System::Gastrointestinal Tract::Intestines::Intestine, Large::Colon
Medical Subject Headings::Diseases::Pathological Conditions, Signs and Symptoms::Signs and Symptoms::Signs and Symptoms, Digestive::Diarrhea
Medical Subject Headings::Chemicals and Drugs::Lipids::Fatty Acids::Fatty Acids, Volatile
Medical Subject Headings::Anatomy::Fluids and Secretions::Feces
Medical Subject Headings::Check Tags::Female
Medical Subject Headings::Anatomy::Fluids and Secretions::Gastrointestinal Contents
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides::Oligopeptides::Glutathione
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Hydrogen-Ion Concentration
Medical Subject Headings::Diseases::Digestive System Diseases::Gastrointestinal Diseases::Intestinal Diseases::Inflammatory Bowel Diseases
Medical Subject Headings::Anatomy::Digestive System::Gastrointestinal Tract::Intestines::Intestinal Mucosa
Medical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Lactobacillales::Lactobacillaceae::Lactobacillus::Lactobacillus fermentum
Medical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Lactobacillales::Lactobacillaceae::Lactobacillus::Lactobacillus reuteri
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Models, Animal
Medical Subject Headings::Anatomy::Musculoskeletal System::Muscles::Muscle, Skeletal
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Physical Examination::Body Constitution::Body Weights and Measures::Organ Size
Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Oxidoreductases::Peroxidases::Peroxidase
Medical Subject Headings::Technology, Industry, Agriculture::Food and Beverages::Food::Dietary Supplements::Probiotics
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Rats
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Rats
Medical Subject Headings::Anatomy::Tissues::Lymphoid Tissue::Spleen
Medical Subject Headings::Chemicals and Drugs::Organic Chemicals::Nitro Compounds::Nitrobenzenes::Trinitrobenzenes::Trinitrobenzenesulfonic Acid
Medical Subject Headings::Chemicals and Drugs::Biological Factors::Intercellular Signaling Peptides and Proteins::Tumor Necrosis Factors::Tumor Necrosis Factor-alpha
Medical Subject Headings::Organisms::Eukaryota::Animals
Medical Subject Headings::Anatomy::Tissues::Lymphoid Tissue::Thymus Gland
Issue Date: 12-Jan-2007
Publisher: Cambridge University Press
Citation: Peran L, Sierra S, Comalada M, Lara-Villoslada F, Bailón E, Nieto A, et al. A comparative study of the preventative effects exerted by two probiotics, Lactobacillus reuteri and Lactobacillus fermentum, in the trinitrobenzenesulfonic acid model of rat colitis. Br. J. Nutr.. 2007 ; 97(1):96-103
Abstract: The intestinal anti-inflammatory effects of two probiotics isolated from breast milk, Lactobacillus reuteri and L. fermentum, were evaluated and compared in the trinitrobenzenesulfonic acid (TNBS) model of rat colitis. Colitis was induced in rats by intracolonic administration of 10 mg TNBS dissolved in 50% ethanol (0.25 ml). Either L. reuteri or L. fermentum was daily administered orally (5 x 10(8) colony-forming units suspended in 0.5 ml skimmed milk) to each group of rats (n 10) for 3 weeks, starting 2 weeks before colitis induction. Colonic damage was evaluated histologically and biochemically, and the colonic luminal contents were used for bacterial studies and for SCFA production. Both probiotics showed intestinal anti-inflammatory effects in this model of experimental colitis, as evidenced histologically and by a significant reduction of colonic myeloperoxidase activity (P<0.05). L. fermentum significantly counteracted the colonic glutathione depletion induced by the inflammatory process. In addition, both probiotics lowered colonic TNFalpha levels (P<0.01) and inducible NO synthase expression when compared with non-treated rats; however, the decrease in colonic cyclo-oxygenase-2 expression was only achieved with L.fermentum administration. Finally, the two probiotics induced the growth of Lactobacilli species in comparison with control colitic rats, but the production of SCFA in colonic contents was only increased when L. fermentum was given. In conclusion, L. fermentum can exert beneficial immunomodulatory properties in inflammatory bowel disease, being more effective than L. reuteri, a probiotic with reputed efficacy in promoting beneficial effects on human health.
Description: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't;
metadata.dc.identifier.doi: 10.1017/S0007114507257770
ISSN: 0007-1145 (Print)
1475-2662 (Online)
Appears in Collections:01- Artículos - Biobanco del Sistema Sanitario Público de Andalucía
01- Artículos - Hospital Virgen de las Nieves

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